金针菇多糖结构解析及其对小鼠 B 淋巴细胞免疫调节活性的研究。
Structural elucidation of a polysaccharide from Flammulina velutipes and its immunomodulation activities on mouse B lymphocytes.
机构信息
National Engineering Research Center of Edible Fungi, Key Laboratory of Applied Mycological Resources and Utilization of Ministry of Agriculture, Shanghai Key Laboratory of Agricultural Genetics and Breeding. Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai, 201403, China.
College of Chinese Traditional Medicine, Jilin Agricultural University, Jilin, Changchun, 130118, China.
出版信息
Sci Rep. 2018 Feb 15;8(1):3120. doi: 10.1038/s41598-018-21375-0.
A novel polysaccharide FVPB2 was purified from fruiting bodies of Flammulina velutipes. Its structure was elucidated by monosaccharide composition and methylation analyses, UV-Visible and FTIR spectroscopy as well as NMR. FVPB2 was a homogeneous heteropolysaccharide (molecular weight ~ 1.50 × 10 Da) containing D-galactose, D-mannose, L-fucose, and D-glucose at molar ratio of 1.9:1.2:1:2.5. In vitro immunomodulatory studies showed FVPB2 induced proliferation of mouse spleen lymphocytes in a dose-dependent manner. The levels of IgM and IgG, secreted by B cells, increased after FVPB2 treatment. So FVPB2 has potential to be a new important immunomodulatory nutraceutical.
一种新型多糖 FVPB2 从金针菇的子实体中被分离出来。其结构通过单糖组成和甲基化分析、紫外可见光谱和傅里叶变换红外光谱以及核磁共振来阐明。FVPB2 是一种均一的杂多糖（分子量约为 1.50×10 Da），含有 D-半乳糖、D-甘露糖、L-岩藻糖和 D-葡萄糖，摩尔比为 1.9:1.2:1:2.5。体外免疫调节研究表明，FVPB2 以剂量依赖的方式诱导小鼠脾淋巴细胞增殖。FVPB2 处理后 B 细胞分泌的 IgM 和 IgG 水平增加。因此，FVPB2 有可能成为一种新的重要的免疫调节营养保健品。
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