Zhao Kai, Zhou Yuzhen, Ahmad Sagheer, Xu Zongda, Li Yushu, Yang Weiru, Cheng Tangren, Wang Jia, Zhang Qixiang
Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, School of Landscape Architecture, Beijing Forestry University, Beijing, China.
Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing, China.
Front Plant Sci. 2018 Feb 1;9:17. doi: 10.3389/fpls.2018.00017. eCollection 2018.
genes are SVP/MADs-box members and supposed to play crucial roles in plant dormancy of perennial species. In , has been previously identified to induce plant dormancy. In the current study, six were cloned in and functionally analyzed in yeast and tobacco to detect the roles of the genes paralogous to . The expression patterns together with sequence similarities indicate that are divided into two sub-clades within SVP group. Moreover, are verified to take part in the development of different plant organs, specifically the flower buds, in some intricate patterns. Furthermore, the PmDAM proteins are found to have special functions by forming corresponding protein complex during the development of flower bud and induction of dormancy. In particular, when PmDAM1 dominating in flower bud in the warm months, the protein complexes are consisted of PmDAM1 itself or with PmDAM2. With the decrease temperatures in the following months, PmDAM6 was found to be highly expressed and gradually changed the complex structure to PmDAM6-protein complex due to strong binding tendencies with PmDAM1 and PmDAM3. Finally, the homodimers of PmDAM6 prevailed to induce the dormancy. The results obtained in the current study highlight the functions of in the tissue development and dormancy, which provide available suggestions for further explorations of protein-complex functions in association with bud growth and dormancy.
基因是SVP/MADs-box成员，被认为在多年生植物的休眠中起关键作用。在[具体研究中]，先前已鉴定出[具体基因]可诱导植物休眠。在本研究中，在[具体植物]中克隆了六个[相关基因]，并在酵母和烟草中进行功能分析，以检测与[具体基因]同源的基因的作用。表达模式和序列相似性表明，[相关基因]在SVP组内分为两个亚分支。此外，已证实[相关基因]以一些复杂的模式参与不同植物器官的发育，特别是花芽。此外，发现PmDAM蛋白在花芽发育和休眠诱导过程中通过形成相应的蛋白复合物而具有特殊功能。特别是，当温暖月份花芽中PmDAM1占主导时，蛋白复合物由PmDAM1自身或与PmDAM2组成。随着接下来几个月温度的降低，发现PmDAM6高度表达，并由于与PmDAM1和PmDAM3的强结合倾向而逐渐将复合物结构改变为PmDAM6-蛋白复合物。最后，PmDAM6的同二聚体占主导以诱导休眠。本研究获得的结果突出了[相关基因]在组织发育和休眠中的功能，为进一步探索与芽生长和休眠相关的蛋白复合物功能提供了有用的建议。
