Hagras Abeer M, Toraih Eman A, Fawzy Manal S
Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
Department of Histology and Cell Biology (Genetics Unit), Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
Biochim Open. 2016 Jul 25;3:19-25. doi: 10.1016/j.biopen.2016.07.003. eCollection 2016 Dec.
NAD-dependent Isocitrate Dehydrogenase (NAD-IDH) could be one of the cell phone radiation targets. Enzyme activity alteration may lead to decline in sperm motility during radio-frequency electromagnetic waves (RF-EMW) exposure. The current case control study aimed to investigate the possible relationship between mitochondrial NAD-IDH activity in human seminal plasma and sperm motility among asthenozoospermic cellular phone users. A total number of ninety idiopathic infertile males referred from the Department of Dermatology and Andrology, were enrolled in this study. NAD-IDH activity was measured in human seminal plasma by spectrophotometer. Computer-aided sperm analysis (CASA) following WHO criteria has been used for semen analyses. The results showed that IDH activity was increased in patients with prolonged cell phone daily use ≥4 h/day. Its level, correlated negatively with either the motility ratio percentages (r = -0.46, < 0.001) or the progressive motility percentages (r = -0.50, < 0.001) in the study groups. The current study suggests that NAD-IDH in human seminal plasma could be one of seminal plasma biomarkers reflecting the mitochondrial function of spermatozoa. Alteration of its level could reflect the defective motility of sperms among some cases of cellular phone users.
NAD 依赖型异柠檬酸脱氢酶（NAD-IDH）可能是手机辐射的靶点之一。酶活性改变可能导致在射频电磁波（RF-EMW）暴露期间精子活力下降。当前的病例对照研究旨在调查弱精子症手机用户的人类精浆中线粒体 NAD-IDH 活性与精子活力之间的可能关系。本研究纳入了总共 90 名从皮肤科和男科转诊来的特发性不育男性。通过分光光度计测量人类精浆中的 NAD-IDH 活性。按照世界卫生组织标准使用计算机辅助精子分析（CASA）进行精液分析。结果显示，每日使用手机时间延长≥4 小时/天的患者中 IDH 活性增加。在研究组中，其水平与活力比率百分比（r = -0.46，<0.001）或进行性活力百分比（r = -0.50，<0.001）均呈负相关。当前研究表明，人类精浆中的 NAD-IDH 可能是反映精子线粒体功能的精浆生物标志物之一。其水平的改变可能反映了某些手机用户中精子活力的缺陷。
