Ferreira Gustavo Dias, Capp Edison, Jauckus Julia, Strowitzki Thomas, Germeyer Ariane
Department of Physiology, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.
Department of Physiology and Pharmacology, Federal University of Pelotas, Campus Universitário Capão do Leão, s/n, Pelotas, RS, 96010-900, Brazil.
Arch Gynecol Obstet. 2018 May;297(5):1175-1179. doi: 10.1007/s00404-018-4719-3. Epub 2018 Feb 15.
The semaphorins are related to angiogenesis and cell proliferation depending on the tissue. The purpose of this study was to assess gene expression of class 3 semaphorin (SEMA3A-F) and protein expression of semaphorin 3A (SEMA3A) within human endometrium throughout the menstrual cycle.
Gene expression of SEMA3A-F was analyzed by real-time PCR (qRT-PCR) and protein expression of SEMA3A was analyzed by ELISA in endometrial biopsies in the proliferative and secretory phase of the menstrual cycle.
Gene expression of SEMA3A, SEMA3C, SEMA3D, and SEMA3E was statistically significant decreased in secretory compared to proliferative phase endometrium (p < 0.05). Accordingly, SEMA3A protein expression in the secretory phase was lower than protein expression in proliferative phase endometrium (p ≤ 0.05).
SEMA3A, 3C, 3D, and 3E are possibly related to cell proliferation in the endometrium, being more expressed in the proliferative phase of the cycle. This finding may stimulate studies of class 3 semaphorins as a possible target for treatment of endometrial pathologies.
根据组织的不同，信号素与血管生成和细胞增殖相关。本研究的目的是评估整个月经周期中人子宫内膜中3类信号素（SEMA3A - F）的基因表达以及信号素3A（SEMA3A）的蛋白表达。
通过实时定量聚合酶链反应（qRT - PCR）分析SEMA3A - F的基因表达，并通过酶联免疫吸附测定（ELISA）分析月经周期增殖期和分泌期子宫内膜活检组织中SEMA3A的蛋白表达。
与增殖期子宫内膜相比，分泌期子宫内膜中SEMA3A、SEMA3C、SEMA3D和SEMA3E的基因表达有统计学意义的下降（p < 0.05）。相应地，分泌期SEMA3A蛋白表达低于增殖期子宫内膜中的蛋白表达（p≤0.05）。
SEMA3A、3C、3D和3E可能与子宫内膜中的细胞增殖相关，在月经周期的增殖期表达更高。这一发现可能会激发对3类信号素作为子宫内膜疾病可能治疗靶点的研究。
