Department of Immunology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Research Center of Otolaryngology Head & Neck Surgery, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Indian J Med Res. 2023 Oct 1;158(4):432-438. doi: 10.4103/ijmr.ijmr_3148_21. Epub 2023 Sep 25.
Semaphorins were initially characterized as axon guidance factors but were subsequently implicated in the regulation of immune responses, angiogenesis, organ formation and a variety of other physiological and developmental functions. Various semaphorins enhance or inhibit tumour progression through different mechanisms. The objective of this study was to assess the expression of various semaphorins and vascular endothelial growth factor (VEGF) gene transcripts as well as the serum level of Sema3A in individuals with laryngeal squamous cell carcinoma (LSCC).
Tissue expression of Sema3A, Sema3C, Sema4D, Sema6D and VEGF was determined in both tumour tissues and tissues around the tumour from 30 individuals with pathologically confirmed LSCC using quantitative real-time PCR. Furthermore, the serum level of Sema3A in these individuals was assessed using enzyme-linked immunosorbent assay.
Sema3C gene transcript showed a significant increase (P=0.001), while Sema4D was observed with a significant decrease in tumour samples compared to non-tumoural tissues (P≤0.01). The expression of the Sema3C gene was found to be associated with the stage of LSCC tumour as it was statistically significant for tumours with stage IV (P<0.01). The serum level of Sema3A was not found to be significant between cases and controls.
Increased expression of Sema3C but decreased expression of Sema4D in tumour tissue of LSCC may introduce these two growth factors as crucial mediators orchestrating tumour growth in individuals with LSCC. This result could open a new vision for the treatment of this malignancy.
最初，信号蛋白被描述为轴突导向因子，但后来它们被认为参与了免疫反应、血管生成、器官形成和多种其他生理和发育功能的调节。各种信号蛋白通过不同的机制促进或抑制肿瘤的进展。本研究旨在评估不同信号蛋白和血管内皮生长因子（VEGF）基因转录物以及 Sema3A 血清水平在喉鳞状细胞癌（LSCC）患者中的表达。
使用定量实时 PCR 检测 30 例经病理证实为 LSCC 的患者肿瘤组织和肿瘤周围组织中 Sema3A、Sema3C、Sema4D、Sema6D 和 VEGF 的组织表达。此外，还使用酶联免疫吸附试验评估这些个体的 Sema3A 血清水平。
与非肿瘤组织相比，肿瘤组织中 Sema3C 基因转录本显著增加（P=0.001），而 Sema4D 则显著减少（P≤0.01）。Sema3C 基因的表达与 LSCC 肿瘤的分期有关，因为对于 IV 期肿瘤，其具有统计学意义（P<0.01）。Sema3A 血清水平在病例和对照组之间没有发现显著差异。
LSCC 肿瘤组织中 Sema3C 的表达增加而 Sema4D 的表达减少，可能使这两种生长因子成为 LSCC 患者肿瘤生长的关键介质。这一结果为这种恶性肿瘤的治疗开辟了新的视野。
