Faculty of Agriculture and Biology, Department of Botany, Warsaw University of Life Sciences (SGGW), Warsaw, Poland.
Faculty of Biotechnology and Horticulture, Institute of Plant Biology and Biotechnology, Unit of Botany and Plant Physiology, University of Agriculture, Krakow, Poland.
Plant Biol (Stuttg). 2018 May;20(3):474-482. doi: 10.1111/plb.12712. Epub 2018 Mar 23.
Information on metallophytes during reclamation of land contaminated with heavy metals is sparse. We investigated the response of D. carthusianorum calamine ecotype to Pb and Cd stress. We focused on in vitro selection of tolerant plant material for direct use in chemically degraded areas. Shoot cultures were treated with various concentrations of Pb or Cd ions. Plantlet status was estimated as micropropagation efficiency, growth tolerance index (GTI) and through physiological analysis. Moreover, determination of plant Pb, Cd and other elements was performed. The application of Pb(NO ) resulted in stronger growth inhibition than application of CdCl . In the presence of Pb ions, a reduction was observed of both, the micropropagation coefficient to 1.1-1.8 and the GTI to 48%. In contrast, Cd ions had a positive influence on tested cultures, expressed as an increase of GTI up to 243% on medium enriched with 1.0 μm CdCl . Moreover, photosynthetic pigment content in shoots cultivated on media with CdCl was higher than in control treatment. The adaptation to Cd was associated with decreased accumulation of phenols in the order: 0.0 μm > 1.0 μm > 3.0 μm > 5.5 μm CdCl . It seems that high tolerance to Cd is related to K uptake, which is involved in antioxidant defence. This work presents an innovative approach to the impact of Cd ions on plant growth and suggests a potential biological role of this metal in species from metalliferous areas.
关于重金属污染土地复垦过程中金属植物的信息很少。我们研究了 D. carthusianorum 钙镁石生态型对 Pb 和 Cd 胁迫的反应。我们专注于从体外选择耐受植物材料，以便直接用于化学退化区域。将芽培养物用不同浓度的 Pb 或 Cd 离子处理。通过微繁殖效率、生长耐量指数（GTI）和生理分析来评估植物苗的状态。此外，还进行了植物 Pb、Cd 和其他元素的测定。与 CdCl 的应用相比，Pb(NO3)2 的应用导致更强的生长抑制。在 Pb 离子存在下，微繁殖系数降低到 1.1-1.8，GTI 降低到 48%。相比之下，Cd 离子对测试培养物有积极影响，表现在富含 1.0μm CdCl 的培养基中 GTI 增加了 243%。此外，在含有 CdCl 的培养基中培养的芽中的光合色素含量高于对照处理。适应 Cd 与酚类物质积累减少有关，顺序为：0.0μm < 1.0μm < 3.0μm < 5.5μm CdCl。高耐 Cd 似乎与 K 摄取有关，K 摄取参与抗氧化防御。这项工作提出了一种新的方法来研究 Cd 离子对植物生长的影响，并暗示了这种金属在富含金属地区物种中的潜在生物学作用。
