Wiszniewska A, Hanus-Fajerska E, Muszyńska E, Smoleń S
Unit of Botany and Plant Physiology, Institute of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in Kraków, Al. 29 Listopada 54, 31-425 Kraków, Poland.
Department of Botany, Faculty of Agriculture and Biology, Warsaw University of Life Sciences (SGGW), Nowoursynowska 159, Building 37, 02-776 Warszawa, Poland.
Water Air Soil Pollut. 2017;228(8):304. doi: 10.1007/s11270-017-3488-0. Epub 2017 Jul 26.
Two species of Pb-adapted shrubs, and , were evaluated in vitro for their tolerance to elevated concentrations of cadmium. Shoot cultures were treated with 0.5, 2.5, and 5.0 μM CdCl for 16 weeks and analyzed for their organogenic response, biomass accretion, pigment content, and macronutrient status. Cadmium accumulation and its root-to-shoot translocation were also determined. In both species, rooted microplantlets, suitable for acclimatization, were obtained in the presence of Cd applied as selection agent. In , low and moderate dose of Cd stimulated multiplication, rooting, and biomass production. Growth tolerance index (GTI) in Cd-treated shoots ranged from 120 to 215%, while in the roots 51-202%. In turn, in Cd-treated proliferation and rooting were inhibited, and GTI for shoots decreased with increasing doses of Cd. However, roots exposed to Cd had higher biomass accretion. Both species accumulated Cd in developed organs, and its content increased with increasing CdCl dose. Interestingly, accumulated higher amounts of Cd in the roots than and immobilized this metal in the root system. On the contrary, translocated some part of accumulated Cd to the shoots, but with low efficiency. In the presence of Cd, maintained macronutrient homeostasis and synthesized higher amounts of phytosynthetic pigments in the shoots. accumulated root biomass, immobilized Cd, and restricted its translocation at the expense of nutrient balance. Considering remediation potential, could be exploited in phytoextraction, while in phytostabilization of polluted substrate.
对两种适应铅的灌木物种[物种名称1]和[物种名称2]进行了体外镉耐受性评估。将茎段培养物用0.5、2.5和5.0 μM CdCl₂处理16周，并分析其器官发生反应、生物量积累、色素含量和大量营养素状况。还测定了镉的积累及其从根到茎的转运。在这两个物种中，在以镉作为选择剂的情况下均获得了适合驯化的生根微型植株。在[物种名称1]中，低剂量和中等剂量的镉刺激了增殖、生根和生物量生产。镉处理茎段的生长耐受指数（GTI）在120%至215%之间，而根中的GTI为51 - 202%。相反，在镉处理的[物种名称2]中，增殖和生根受到抑制，茎段的GTI随镉剂量增加而降低。然而，暴露于镉的根具有更高的生物量积累。两个物种在发育的器官中都积累了镉，其含量随CdCl₂剂量增加而增加。有趣的是，[物种名称1]在根中积累的镉比[物种名称2]多，并将这种金属固定在根系中。相反，[物种名称2]将部分积累的镉转运到茎中，但效率较低。在有镉存在的情况下，[物种名称1]维持了大量营养素的稳态，并在茎中合成了更多的光合色素。[物种名称2]积累了根生物量，固定了镉，并以营养平衡为代价限制了其转运。考虑到修复潜力，[物种名称1]可用于植物提取，而[物种名称2]可用于污染基质的植物稳定化。
