Vieira J N, Feijó A M, Bueno M E, Gonçalves C L, Lund R G, Mendes J F, Villarreal J P V, Villela M M, Nascente P S
Laboratório de Parasitologia, Instituto de Biologia, Departamento de Microbiologia e Parasitologia, Universidade Federal de Pelotas - UFPEL, Campus universitário Capão do Leão, s/n, Prédio 18, Sala 3, CEP 96010-900, Pelotas, RS, Brazil.
Departamento de Odontologia Restauradora, Faculdade de Medicina Dentária, Universidade Federal de Pelotas - UFPEL, Rua Gonçalves Chaves, nº 457, Centro, CEP 96020-080, Pelotas, RS, Brazil.
Braz J Biol. 2018 Nov;78(4):644-652. doi: 10.1590/1519-6984.169623. Epub 2018 Feb 15.
The aim of this study was to evaluate the frequency of Candida species between a non-hospitalized and a hospitalized population. For this purpose, samples of saliva were sampled through sterile swabs, moistened in peptone water and rubbed in the oral cavity of 140 individuals, from which, 70 were hospitalized patients from the Medical Clinic of a Teaching Hospital and the other 70 were non-hospitalized subjects. All saliva samples were plated in Sabouraud Dextrose agar added with Chloramphenicol and incubated at 36 °C for 48 hours. The morphology identification was performed through macroscopic and microscopic characterization, the CHROMagar Candida medium and the VITEK® system Yeast Biochemical Card (bio Mérieux SA, France). The results showed a colonization of Candida spp. in 85.7% the hospitalized individuals, where the species found were C. albicans (60%), C. tropicalis (23.4%), C. krusei (3.3%) and Candida spp. (13.3%). In the non-hospitalized individuals the colonization by Candida spp was 47.1%, and the species found were: C. albicans (45.5%), C.krusei (9.1%), C. guilliermondii (9.1% %), C. tropicalis (3.0%), C. famata (3.0%) and Candida spp. (30.3%). In spite of their presence in oral cavity in both groups, Candida spp. was more frequently isolated in hospitalized individuals, who were 6.73 times more likely to have this fungus in the oral cavity and were 3.88 times more likely to have Candida albicans.
本研究的目的是评估非住院人群和住院人群中念珠菌属的感染频率。为此，通过无菌拭子采集唾液样本，将拭子在蛋白胨水中浸湿后在140名个体的口腔中擦拭，其中70名是一家教学医院内科诊所的住院患者，另外70名是非住院受试者。所有唾液样本均接种于添加氯霉素的沙氏葡萄糖琼脂平板上，在36℃孵育48小时。通过宏观和微观特征、CHROMagar念珠菌培养基和VITEK®系统酵母生化鉴定卡（法国生物梅里埃公司）进行形态学鉴定。结果显示，85.7%的住院个体有念珠菌属定植，其中发现的菌种为白色念珠菌（60%）、热带念珠菌（23.4%）、克柔念珠菌（3.3%）和念珠菌属（13.3%）。在非住院个体中，念珠菌属的定植率为47.1%，发现的菌种为：白色念珠菌（45.5%）、克柔念珠菌（9.1%）、季也蒙念珠菌（9.1%）、热带念珠菌（3.0%）、法塔念珠菌（3.0%）和念珠菌属（30.3%）。尽管两组人群口腔中均有念珠菌属存在，但住院个体中念珠菌属的分离频率更高，其口腔中有这种真菌的可能性是非住院个体的6.73倍，感染白色念珠菌的可能性是非住院个体的3.88倍。
