Madhavan P, Jamal F, Chong P P, Ng K P
Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, University Putra Malaysia, Serdang, Selangor, Malaysia.
Trop Biomed. 2011 Aug;28(2):269-74.
The objective of our study was to study the effectiveness of CHROMagar Candida™ as the primary identification method for various clinical Candida isolates, other than the three suggested species by the manufacturer. We studied 34 clinical isolates which were isolated from patients in a local teaching hospital and 7 ATCC strains. These strains were first cultured in Sabouraud dextrose broth (SDB) for 36 hours at 35ºC, then on CHROMagar plates at 30ºC, 35ºC and 37ºC. The sensitivity of this agar to identify Candida albicans, Candida dubliniensis, Candida tropicalis, Candida glabrata, Candida rugosa, Candida krusei and Candida parapsilosis ranged between 25 and 100% at 30ºC, 14% and 100% at 35ºC, 56% and 100% at 37ºC. The specificity of this agar was 100% at 30ºC, between 97% and 100% at 35ºC, 92% and 100% at 37ºC. The efficiency of this agar ranged between 88 and 100% at 30ºC, 83% and 100% at 35ºC, 88% and 100% at 37ºC. Each species also gave rise to a variety of colony colours ranging from pink to green to blue of different colony characteristics. Therefore, the chromogenic agar was found to be useful in our study for identifying clinical Candida isolates.
我们研究的目的是探究除制造商建议的三种念珠菌外，CHROMagar Candida™作为各种临床念珠菌分离株主要鉴定方法的有效性。我们研究了从当地一家教学医院的患者中分离出的34株临床分离株和7株美国典型培养物保藏中心（ATCC）菌株。这些菌株首先在沙氏葡萄糖肉汤（SDB）中于35ºC培养36小时，然后在30ºC、35ºC和37ºC的CHROMagar平板上培养。该琼脂在30ºC鉴定白色念珠菌、都柏林念珠菌、热带念珠菌、光滑念珠菌、皱落念珠菌、克柔念珠菌和近平滑念珠菌的敏感性在25%至100%之间，在35ºC为14%至100%，在37ºC为56%至100%。该琼脂在30ºC的特异性为100%，在35ºC为97%至100%，在37ºC为92%至100%。该琼脂在30ºC的效率在88%至100%之间，在35ºC为83%至100%，在37ºC为88%至100%。每个菌种还产生了从粉色到绿色再到蓝色的各种菌落颜色，菌落特征各异。因此，在我们的研究中发现显色琼脂对于鉴定临床念珠菌分离株很有用。
