San-Millán R, Ribacoba L, Pontón J, Quindós G
Departamento de Inmunología, Microbiología y Parasitología, Facultad de Medicina y Odontología, Universidad del País Vasco-Euskal Herriko Unibertsitatea, Bilbao, Spain.
Eur J Clin Microbiol Infect Dis. 1996 Feb;15(2):153-8. doi: 10.1007/BF01591489.
CHROMagar Candida (CHROMagar, France) was evaluated as a medium for the presumptive identification and isolation of yeasts using 1,537 isolates of medically important yeasts, including 970 Candida albicans, 165 Candida parapsilosis, 131 Candida glabrata, 62 Candida guilliermondii, 35 Candida krusei, 32 Candida tropicalis, 31 Rhodotorula rubra, 23 Trichosporon spp. (17 Trichosporon beigelii), 17 Candida famata, 16 Candida pelliculosa, 10 Pichia etchelsii, 10 Saccharomyces cerevisiae, 8 Candida lusitaniae, 7 Cryptococcus spp., and 20 isolates of other Candida spp. After 48 h of incubation at 37 degrees C, the sensitivity and specificity were, respectively, 99% and 100% for Candida albicans, 93.8% and 99.1% for Candida tropicalis, and 100% and 100% for Candida krusei. In addition to colony color, other colony characteristics were important for identification of some species, such as rough colonies in Candida krusei isolates or the halo around the colonies of Candida tropicalis. A great variety of colors was observed among species other than Candida albicans, Candida tropicalis, and Candida krusei. For identification purposes, CHROMagar Candida medium has an accuracy similar to that of germ-tube tests and chlamydospore development tests for Candida albicans and to that of the ATB ID32C kit (API, bioMérieux, France) for Candida tropicalis and Candida krusei.
使用1537株具有医学重要性的酵母菌株，包括970株白色念珠菌、165株近平滑念珠菌、131株光滑念珠菌、62株季也蒙念珠菌、35株克柔念珠菌、32株热带念珠菌、31株深红酵母、23株丝孢酵母属（17株白吉利丝孢酵母）、17株无名念珠菌、16株膜醭念珠菌、10株埃切尔斯毕赤酵母、10株酿酒酵母、8株葡萄牙念珠菌、7株隐球菌属以及20株其他念珠菌属菌株，对法国CHROMagar公司生产的念珠菌显色培养基（CHROMagar Candida）作为一种用于酵母初步鉴定和分离的培养基进行了评估。在37℃孵育48小时后，白色念珠菌的敏感性和特异性分别为99%和100%，热带念珠菌为93.8%和99.1%，克柔念珠菌为100%和100%。除菌落颜色外，其他菌落特征对某些菌种的鉴定也很重要，例如克柔念珠菌分离株中的粗糙菌落或热带念珠菌菌落周围的晕圈。在白色念珠菌、热带念珠菌和克柔念珠菌以外的菌种中观察到了多种颜色。出于鉴定目的，CHROMagar念珠菌培养基对于白色念珠菌的准确性与芽管试验和厚壁孢子形成试验相似，对于热带念珠菌和克柔念珠菌的准确性与ATB ID32C试剂盒（法国生物梅里埃公司API）相似。
