Dawé Amadou, Mbiantcha Marius, Yakai Fawai, Jabeen Almas, Ali Muhammad Shaiq, Lateef Mehreen, Ngadjui Bonaventure Tchaleu
Department of Chemistry, Higher Teachers Training College, University of Maroua, P.O. Box 55, Maroua, Cameroon, Phone: +00237-694457325, Fax: +00237-22 29 31 12.
Department of Animal Biology, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon.
Z Naturforsch C J Biosci. 2018 Apr 25;73(5-6):211-219. doi: 10.1515/znc-2017-0166.
Despite the well-documented benefits of Combretum fragrans in Cameroon, only few scientific works have been done on it. In this study we isolated eight compounds from the leaves extract of C. fragrans: velutin (1), belamcanidin (2), cirsilineol (3), cirsimaritin (4), 3β-acetoxy-20,24-epoxy-11,25-hydroxy-dammarane (5), combretin A (6), combretin B (7) and a mixture of arjunolic acid (8a) and asiatic acid (8b). Compounds 6 and 7 presented potent anti-inflammatory, antioxidant and antidiabetic activities. Compounds 1, 3, 5 and the mixture of 8a and 8b were significantly active, and compounds 2 and 4 presented moderate activity for reactive oxygen species inhibitory and free-radical scavenging. All compounds were isolated using chromatographic techniques; their structures were elucidated by spectroscopic techniques and their spectroscopic data compared with those of the literature. Anti-inflammatory activity was evaluated via the oxidative burst assay using a luminol-amplified chemiluminescence technique, antioxidant activity by free-radical scavenging activity (DPPH) and antidiabetic activity via α-glucosidase inhibition. All of the isolated compounds (1-8) were reported to exhibit significant antioxidant activity. Compounds 1, 3, and 5-8 exhibited potent chemiluminescence inhibition effect, and only compounds 6 and 7 inhibited α-glucosidase. Thus, C. fragrans can be used as an effective natural source of anti-inflammatory, antioxidant and antidiabetic compounds.
尽管在喀麦隆康氏风车子的益处已有充分记录，但对其开展的科学研究却很少。在本研究中，我们从康氏风车子叶提取物中分离出8种化合物：绒毛苷（1）、贝拉坎定（2）、环稀醚萜醇（3）、 cirsimaritin（4）、3β - 乙酰氧基 - 20,24 - 环氧 - 11,25 - 羟基 - 达玛烷（5）、风车子亭A（6）、风车子亭B（7）以及熊果酸（8a）和积雪草苷（8b）的混合物。化合物6和7具有强大的抗炎、抗氧化和抗糖尿病活性。化合物1、3、5以及8a和8b的混合物具有显著活性，化合物2和4对活性氧抑制和自由基清除呈现中等活性。所有化合物均采用色谱技术分离；其结构通过光谱技术阐明，并将其光谱数据与文献数据进行比较。通过使用鲁米诺增强化学发光技术的氧化爆发试验评估抗炎活性，通过自由基清除活性（DPPH）评估抗氧化活性，通过α - 葡萄糖苷酶抑制评估抗糖尿病活性。据报道，所有分离出的化合物（1 - 8）均具有显著的抗氧化活性。化合物1、3以及5 - 8表现出强大的化学发光抑制作用，只有化合物6和7抑制α - 葡萄糖苷酶。因此，康氏风车子可作为抗炎、抗氧化和抗糖尿病化合物的有效天然来源。
