Bahadori Ehsan Shariat, Sadraei Javid, Dalimi Abdolhosein
Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
J Parasit Dis. 2019 Sep;43(3):360-367. doi: 10.1007/s12639-019-01099-2. Epub 2019 Feb 28.
Rodents and stray cats are the sources of many parasitic infections including for other animals and human. Toxoplasmosis has a wide range of laboratory factors in its intermediate and definite hosts. Regarding the importance of rodents and stray cats as the hosts that spread the , it is necessary to obtain comprehensive information about these animals in the life cycle of . The objective was to investigate the new prevalence of toxoplasmosis among target animals in Iran, using GRA6 gene in combinacion with ELISA avidity. In this study, 286 rodents and 210 stray cats were collected and their heart tissues extracted to obtain DNA, blood samples and IgG Ab of parasite. We detected the positive tissue samples in our study by the nested-PCR method. Then, we examined IgG ELISA avidity for assessment of toxoplasmosis among rodents and stray cats. This study, was conducted in January to March 2017, based on the prevalence study. The findings revealed that 246/286 (86.01%) of rodents and 180/210 (85.71%) of stray cats were positive by IgG ELISA avidity methods. moreover, 68 rodents samples and 38 stray cats samples were positive concerning the GRA6 gene; and these positive samples were at intermediate levels for IgG avidity. We concluded that the new prevalence of toxoplasmosis among rodents and stray cats was at high levels, using the serologic method in Northeast of Iran and the results of quantitative ELISA avidity were as the same as those of the nested-PCR for detecting recent toxoplasmosis in these hosts.
啮齿动物和流浪猫是包括对其他动物和人类在内的许多寄生虫感染的源头。弓形虫病在其中间宿主和终末宿主中有广泛的实验室因素。鉴于啮齿动物和流浪猫作为传播弓形虫病宿主的重要性，有必要获取关于这些动物在弓形虫病生命周期中的全面信息。目的是利用GRA6基因结合ELISA亲和力来调查伊朗目标动物中弓形虫病的新患病率。在本研究中，收集了286只啮齿动物和210只流浪猫，并提取它们的心脏组织以获得DNA、血液样本和弓形虫的IgG抗体。我们通过巢式PCR方法检测了本研究中的阳性组织样本。然后，我们检测了弓形虫IgG ELISA亲和力，以评估啮齿动物和流浪猫中的弓形虫病。本研究基于患病率研究于2017年1月至3月进行。研究结果显示，通过IgG ELISA亲和力方法，286只啮齿动物中有246只（86.01%）呈阳性，210只流浪猫中有180只（85.71%）呈阳性。此外，68份啮齿动物样本和38份流浪猫样本的GRA6基因呈阳性；并且这些阳性样本的IgG亲和力处于中等水平。我们得出结论，在伊朗东北部使用血清学方法，啮齿动物和流浪猫中弓形虫病的新患病率处于高水平，并且定量ELISA亲和力的结果与巢式PCR检测这些宿主近期弓形虫病的结果相同。
