Research Institute of Surface Engineering, Taiyuan University of Technology, Taiyuan, China; Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, 4059, Australia; Australia-China Centre for Tissue Engineering and Regenerative Medicine, Queensland University of Technology, Brisbane, 4059, Australia.
Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, 4059, Australia; Australia-China Centre for Tissue Engineering and Regenerative Medicine, Queensland University of Technology, Brisbane, 4059, Australia.
Biomaterials. 2018 Apr;162:154-169. doi: 10.1016/j.biomaterials.2018.02.010. Epub 2018 Feb 6.
A multifaceted coating for hard tissue implants, with favorable osteogenesis, angiogenesis, and osteoimmunomodulation abilities, would be of great value since it could improve osseointegration and alleviate prosthesis loosening. However, to date there are few coatings that fully satisfy these criteria. Herein we describe a microporous TiO coating decorated with hydroxyapatite (HA) nanoparticles that is generated by micro-arc oxidation of pure titanium (Ti) and followed annealing. By altering the annealing temperature, it is possible to simultaneously tune the coating's physical (morphology and wettability) and chemical (composites and crystallinity) properties. A coating produced with micro-arc oxidization (MAO) with an annealing temperature of 650 °C (MAO-650) exhibits numerous favorable physicochemical properties, such as hybrid micro-nano morphology, superhydrophilicity, and highly crystalline HA nanoparticles. In vitro experiments reveal that the MAO-650 coating not only supports proliferation and differentiation of both osteoblasts and endothelial cells, but also inhibits the inflammatory response of macrophages and enables a favorable osteoimmunomodulation to facilitate osteo/angio-genesis. In vivo evaluation mirrors these results, and shows that the MAO-650 coating results in ameliorative osseointegration when compared with the pristine MAO coating. These data highlight the profound effect of surface physicochemical properties on the regulation of osteo/angio-genesis and osteoimmunomodulation in the enhancement of osseointegration.
一种具有多方面特性的硬组织植入物涂层，具有良好的成骨、血管生成和骨免疫调节能力，将具有重要价值，因为它可以改善骨整合并减轻假体松动。然而，迄今为止，很少有涂层完全满足这些标准。本文描述了一种通过纯钛（Ti）的微弧氧化（MAO）和随后的退火处理生成的多孔 TiO2 涂层，该涂层用纳米羟基磷灰石（HA）进行了修饰。通过改变退火温度，可以同时调节涂层的物理（形貌和润湿性）和化学（复合材料和结晶度）性质。退火温度为 650°C 的微弧氧化（MAO）处理生成的涂层（MAO-650）具有许多有利的物理化学性质，例如混合微纳米形貌、超亲水性和高度结晶的 HA 纳米颗粒。体外实验表明，MAO-650 涂层不仅支持成骨细胞和内皮细胞的增殖和分化，而且还抑制巨噬细胞的炎症反应，并实现有利的骨免疫调节，以促进骨/血管生成。体内评估反映了这些结果，并表明与原始 MAO 涂层相比，MAO-650 涂层可改善骨整合。这些数据强调了表面物理化学性质对调节骨/血管生成和骨免疫调节以增强骨整合的深远影响。
