Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USA.
Janelia Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, USA.
Curr Opin Neurobiol. 2018 Jun;50:101-108. doi: 10.1016/j.conb.2018.01.003. Epub 2018 Feb 16.
The ultimate goal of neuroscience is to relate the complex activity of cells and cell-networks to behavior and cognition. This requires tools and techniques to visualize neuronal activity. Fluorescence microscopy is an ideal tool to measure activity of cells in the brain due to the high sensitivity of the technique and the growing portfolio of optical hardware and fluorescent sensors. Here, we give a chemist's perspective on the recent progress of fluorescent activity indicators that enable the measurement of cellular events in the living brain. We discuss advances in both chemical and genetically encoded sensors and look forward to hybrid indicators, which incorporate synthetic organic dyes into genetically encoded protein constructs.
神经科学的最终目标是将细胞和细胞网络的复杂活动与行为和认知联系起来。这需要工具和技术来可视化神经元活动。荧光显微镜是测量大脑中细胞活性的理想工具，因为该技术具有高灵敏度，并且光学硬件和荧光传感器的组合不断增加。在这里，我们从化学家的角度介绍了荧光活性指示剂的最新进展，这些指示剂使我们能够测量活脑中的细胞事件。我们讨论了化学和基因编码传感器的进展，并期待着将合成有机染料纳入基因编码蛋白结构中的混合指示剂。
