Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, USA.
Center for Metagenomics and Microbiome Research, Baylor College of Medicine, Houston, TX, USA.
Methods Mol Biol. 2024;2751:33-46. doi: 10.1007/978-1-0716-3617-6_3.
Recent technological advances in microscopy have facilitated novel approaches to investigate host-pathogen interactions. In particular, improvements in both microscope hardware and engineered biosensors have helped to overcome barriers to live-cell imaging with fluorescence microscopy. Live fluorescent microscopy allows for the detection of discrete signaling events and protein localization, improving our ability to assess the effects of pharmacologic agents, microbes, or infection with high temporal resolution. Here we describe a protocol for long-term live-cell fluorescence imaging of virus infected cell lines.
近年来显微镜技术的进步为研究宿主-病原体相互作用提供了新的方法。特别是，显微镜硬件和工程生物传感器的改进有助于克服荧光显微镜活细胞成像的障碍。活荧光显微镜可用于检测离散的信号事件和蛋白质定位，提高我们评估药理试剂、微生物或感染对高时间分辨率的影响的能力。在这里，我们描述了一种用于病毒感染细胞系的长期活细胞荧光成像的方案。
