Chen Lin, Wang Huagui, Guo Tongsheng, Xiao Chaohui, Liu Licheng, Zhang Xiaoguang, Liu Bo, Li Peiran, Liu Aixia, Li Bo, Li Boan, Mao Yuanli
Medical University of PLA, Beijing, China; Center for Clinical Laboratory, 302 Hospital of PLA, Beijing, China.
Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China; Beijing Macro & Micro Test Biotech Co., Ltd, Beijing, China.
J Immunol Methods. 2018 May;456:23-27. doi: 10.1016/j.jim.2018.02.005. Epub 2018 Feb 15.
Dengue fever is caused by the dengue virus (DENV), and DENV1 is the prevalent epidemic serotype in south China. A new lateral flow assay (LFA) based on a near-infrared (NIR) fluorescent dye was developed to detect anti-DENV1 IgG antibodies. DyLight-800 was used as the marker conjugated to goat anti-human IgG antibodies, and recombinant dengue type 1 envelope protein was used as the capture protein on the test line. Twenty samples from patients infected with DENV1 and 160 negative controls were analyzed using this new NIR-LFA. The results of the NIR-LFA were compared with the results of Panbio Dengue IgG ELISA and the Dengue Duo IgM/IgG Cassette. Nineteen confirmed DENV1-positive samples were identified by NIR-LFA, giving 95% (19/20) sensitivity. No significant differences existed in the results when the 20 primary clinical samples were analyzed using NIR-LFA, Panbio ELISA, and the Dengue Duo Cassette. However, NIR-LFA had a lower limit of detection than IgG ELISA and Duo IgM/IgG Cassette did when analyzing a 2-fold dilution series of the 19 samples positively identified by NIR-LFA. When incorporated with an NIR POCT device, the new NIR-LFA was rapid, easy to use, and highly sensitive in detecting DENV1, and has potential for application to clinical diagnosis.
登革热由登革病毒（DENV）引起，DENV1是中国南方流行的血清型。开发了一种基于近红外（NIR）荧光染料的新型侧向流动分析法（LFA）来检测抗DENV1 IgG抗体。DyLight-800用作与山羊抗人IgG抗体偶联的标记物，重组登革热1型包膜蛋白用作检测线上的捕获蛋白。使用这种新型NIR-LFA分析了20份感染DENV1患者的样本和160份阴性对照。将NIR-LFA的结果与Panbio登革热IgG ELISA和登革热双联IgM/IgG检测卡的结果进行比较。NIR-LFA鉴定出19份确诊的DENV1阳性样本，灵敏度为95%（19/20）。使用NIR-LFA、Panbio ELISA和登革热双联检测卡分析20份原始临床样本时，结果无显著差异。然而，在分析由NIR-LFA阳性鉴定的19份样本的2倍稀释系列时，NIR-LFA的检测下限低于IgG ELISA和双联IgM/IgG检测卡。与NIR即时检测设备结合使用时，新型NIR-LFA检测DENV1快速、易用且高度灵敏，具有临床诊断应用潜力。
