Laboratorio de Genética e Inmunología Molecular, Instituto de Biología, Facultad de Ciencias, Pontificia Universidad Católica de Valparaíso, Campus Curauma, Valparaíso, Chile; Unidad de Microscopía Confocal, Núcleo Biotecnología Curauma, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile.
Laboratorio de Genética e Inmunología Molecular, Instituto de Biología, Facultad de Ciencias, Pontificia Universidad Católica de Valparaíso, Campus Curauma, Valparaíso, Chile; Laboratorio de Patógenos Acuícolas, Núcleo Biotecnología Curauma, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile; Unidad de Microscopía Confocal, Núcleo Biotecnología Curauma, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile.
Microb Pathog. 2018 Apr;117:219-224. doi: 10.1016/j.micpath.2018.02.029. Epub 2018 Feb 16.
The infectious salmon anemia virus (ISAV) is an aquatic pathogen that is a member of the Orthomyxoviridae family with lethal hemorrhagic potential. Although it affects other species of salmonid fish, ISAV only causes disease in Atlantic salmon (Salmo salar) specimens in sea water. In spite of the fact that the virus has been described as enveloped with icosahedral symmetry, viral like particles with anomalous morphology have been observed in field samples, this we have not been able to recover then in adequate quantities for full demonstration. We report a procedure to concentrate and recover these novel forms of the virus, comparing two cell lines from different origins, demonstrating that these forms were preferentially expressed in cells of epithelial origin.
传染性鲑鱼贫血病毒（ISAV）是一种水生病原体，属于正粘病毒科，具有致命性出血潜力。尽管它会影响其他鲑鱼科鱼类，但只有在海水中的大西洋鲑（Salmo salar）标本中才会引发疾病。尽管该病毒已被描述为具有二十面体对称性的包膜病毒，但在野外样本中观察到了具有异常形态的类似病毒颗粒，但我们尚未能够从中回收足够数量的病毒颗粒以进行充分展示。我们报告了一种浓缩和回收这些新型病毒的方法，比较了来自不同来源的两种细胞系，证明这些形式在源自上皮细胞的细胞中优先表达。
