Calvo-Guirado José Luis, Cegarra Del Pino Pilar, Sapoznikov Lari, Delgado Ruiz Rafael Arcesio, Fernández-Domínguez Manuel, Gehrke Sérgio Alexandre
Oral Surgery and Oral Implantology Department, Universidad Católica San Antonio de Murcia, Murcia, Spain; International Dentistry Research Cathedra, Faculty of Health Sciences, Universidad Católica San Antonio de Murcia, Murcia, Spain.
Faculty of Health Sciences, Universidad Católica San Antonio de Murcia, Murcia, Spain.
Ann Anat. 2018 May;217:14-23. doi: 10.1016/j.aanat.2017.12.010. Epub 2018 Feb 15.
To investigate freshly extracted dental particulate used to graft post-extraction sockets in dogs, comparing new bone formation at experimental and control sites.
Bilateral premolars P2, P3, P4 and first mandibular molars were extracted atraumatically from six American Fox Hound dogs. The teeth were ground immediately using a 'Smart Dentin Grinder'. The dentin particulate was sieved to ensure a grain size of 300-1200μm and immersed in an alcohol cleanser to dissolve organic debris and bacteria, followed by washing in sterile saline buffer solution. The animals were divided into two groups randomly: group 'A' (control) samples were left to heal without any extraction socket grafting procedure; group 'B' (experimental) sockets were filled with the autogenous dentin particulate graft. The rate of tissue healing and the quantity of bone formation were evaluated using histological and histomorphometric analyses at 60 and 90 days post-grafting. The type of bone generated was categorized as woven (immature bone) or lamellar bone (mature bone).
Substantially more bone formation was found in Group B (experimental) than Group A (control) at 60 and 90 days (p<0.05). Less immature bone was identified in the dentin grafted group (25.7%) than the control group (55.9%) [corrected]. Similar differences were also observed at 90 days post grafting.
Autogenous dentin particulate grafted immediately after extractions may be considered a useful biomaterial for socket preservation, protecting both buccal and lingual plates, generating large amounts of new woven bone formation after 60 days, and small amounts of lamellar bone after 90 days healing.
研究用于移植犬拔牙窝的新鲜提取牙质颗粒，比较实验部位和对照部位的新骨形成情况。
从6只美国猎狐犬身上无创拔除双侧前磨牙P2、P3、P4和第一下颌磨牙。牙齿立即用“智能牙本质研磨机”研磨。将牙本质颗粒过筛以确保粒度为300 - 1200μm，然后浸入酒精清洁剂中以溶解有机碎片和细菌，接着用无菌生理盐水缓冲液冲洗。动物被随机分为两组：“A”组（对照组）样本不进行任何拔牙窝移植程序任其愈合；“B”组（实验组）拔牙窝填充自体牙本质颗粒移植物。在移植后60天和90天，使用组织学和组织形态计量学分析评估组织愈合率和骨形成量。生成的骨类型分为编织骨（不成熟骨）或板层骨（成熟骨）。
在60天和90天时，“B”组（实验组）的骨形成明显多于“A”组（对照组）（p<0.05）。牙本质移植组中识别出的不成熟骨（25.7%）比对照组（55.9%）[校正后]少。在移植后90天也观察到类似差异。
拔牙后立即移植自体牙本质颗粒可被视为一种用于牙槽窝保存的有用生物材料，可保护颊侧和舌侧骨板，在60天后产生大量新的编织骨形成，在愈合90天后产生少量板层骨。
