College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China.
College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China.
Int J Biol Macromol. 2018 Jul 1;113:20-28. doi: 10.1016/j.ijbiomac.2018.01.223. Epub 2018 Feb 15.
A new Athelia rolfsii exopolysaccharides (AEPS) were purified by Sephacryl S-300 and S-200. The physicochemical characteristics of AEPS fractions were assayed by HPGPC and GC methods. The structures of AEPS and AEPS‑zinc complex were characterized by SEM, FTIR and NMR. Moreover, the bioactivities of complex were also evaluated by experiments in vitro and in vivo. AEPSI consisted of glucose, galacturonic acid, talose, galactose, mannose and xylose, the relative contents of them were 24.74, 19.60, 33.65, 8.77, 7.97 and 5.28%, respectively. AEPSII consisted of glucose, inositol, galacturonic acid, ribitol, gluconic acid, talose and xylose, whose relative contents were 36.06, 21.21, 12.78, 11.07, 6.58, 5.45 and 6.82%, respectively. The Mw and Mn of AEPSI were 6.1324×10 and 1.4218×10Da, those of AEPSII were 517 and 248Da. SEM observations showed that microstructures of AEPS and AEPS‑zinc complex were obviously different both in size and shape. FTIR and NMR analysis indicated that AEPS might chelate with zinc ion through hydroxy and carboxy group. In vitro experiments showed that AEPS‑zinc complex had a good bioavailability, in vivo experiments showed that it had good effect on improving zinc deficiency and antioxidant activities, which suggested that it could be used as zinc supplementation with high antioxidant activities.
一种新的阿地白介素 rolfsii 胞外多糖 (AEPS) 通过 Sephacryl S-300 和 S-200 进行纯化。通过 HPGPC 和 GC 方法测定 AEPS 级分的理化特性。通过 SEM、FTIR 和 NMR 对 AEPS 和 AEPS-锌络合物的结构进行了表征。此外，还通过体外和体内实验评价了络合物的生物活性。AEPSI 由葡萄糖、半乳糖醛酸、塔罗糖、半乳糖、甘露糖和木糖组成，其相对含量分别为 24.74%、19.60%、33.65%、8.77%、7.97%和 5.28%。AEPSII 由葡萄糖、肌醇、半乳糖醛酸、核糖醇、葡萄糖酸、塔罗糖和木糖组成，其相对含量分别为 36.06%、21.21%、12.78%、11.07%、6.58%、5.45%和 6.82%。AEPSI 的 Mw 和 Mn 分别为 6.1324×10 和 1.4218×10Da，AEPSII 的 Mw 和 Mn 分别为 517 和 248Da。SEM 观察表明，AEPS 和 AEPS-锌络合物的微观结构在大小和形状上明显不同。FTIR 和 NMR 分析表明，AEPS 可能通过羟基和羧基与锌离子螯合。体外实验表明，AEPS-锌络合物具有良好的生物利用度，体内实验表明，它对改善锌缺乏和抗氧化活性有良好的效果，这表明它可以作为具有高抗氧化活性的锌补充剂使用。
