Institute of Horticulture, Zhejiang Academy of Agricultural Science, Hangzhou 310021, PR China.
Institute of Horticulture, Zhejiang Academy of Agricultural Science, Hangzhou 310021, PR China.
Int J Biol Macromol. 2019 May 15;129:861-868. doi: 10.1016/j.ijbiomac.2019.02.082. Epub 2019 Feb 14.
The polysaccharide (PPB-2) was extracted and purified from the fruiting body of artificially cultivated Phellinus baumii by an acid-chlorite delignification method. The in vitro hypoglycemic and in vivo hepatoprotective activities of PPB-2 were investigated. FT-IR spectra, NMR spectral analysis, and scanning electron microscopy were used to investigate the structural characterization of PPB-2. PPB-2 exhibited higher α-glucosidase and α-amylase inhibitory activities and showed significant inhibitory effects against the diffusion of glucose. Furthermore, the administration of PPB-2 to mice significantly prevented the increase of serum alanine aminotransferase and aspartate aminotransferase activities, as well as the increase in total cholesterol, triglycerides, and total bilirubin, and hepatic malondialdehyde levels, and PPB-2 enhanced the activities of superoxide dismutase and catalase, as well as the glutathione levels. The results suggest that PPB-2 may be suitable as a functional food with hypoglycemic and hepatoprotective activities.
从人工栽培的鲍氏层孔菌子实体中提取和纯化多糖（PPB-2），采用酸-次氯酸盐脱木质素法。研究了 PPB-2 的体外降血糖和体内保肝活性。利用傅里叶变换红外光谱、核磁共振波谱分析和扫描电子显微镜对 PPB-2 的结构特征进行了研究。PPB-2 表现出更高的α-葡萄糖苷酶和α-淀粉酶抑制活性，并对葡萄糖扩散表现出显著的抑制作用。此外，给予 PPB-2 可显著预防血清丙氨酸氨基转移酶和天冬氨酸氨基转移酶活性升高，总胆固醇、甘油三酯和总胆红素以及肝丙二醛水平升高，并增强超氧化物歧化酶和过氧化氢酶活性，以及谷胱甘肽水平。结果表明，PPB-2 可能适合作为具有降血糖和保肝活性的功能性食品。
