Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Kraków, Poland.
Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Kraków, Poland.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 May 5;196:262-267. doi: 10.1016/j.saa.2018.02.027. Epub 2018 Feb 7.
Adsorption or immobilization of proteins on synthetic surfaces is a key issue in the context of the biocompatibility of implant materials, especially those intended for the needs of cardiac surgery but also for the construction of biosensors or nanomaterials used as drug carriers. The subject of research was the analysis of Raman spectra of two types of fibrous carbon nanomaterials, of great potential for biomedical applications, incubated with human serum albumin (HSA). The first nanomaterial has been created on the layer of MWCNTs deposited by electrophoretic method (EPD) and then covered by thin film of pyrolytic carbon introduced by chemical vapor deposition process (CVD). The second material was formed from carbonized nanofibers prepared via electrospinning (ESCNFs) of polyacrylonitrile (PAN) precursor and then covered with pyrolytic carbon (CVD). The G-band blue-shift towards the position of about 1600cm, observed for both studied surfaces, clearly indicates the albumin (HSA) adhesion to the surface. The G and G' (2D) peak shift was employed to assess the stress build up on the carbon nanomaterials. The surface nano- and micro-topography as well as the method of ordering the carbon nanomaterial has a significant influence on the mode of surface-protein interaction.
蛋白质在合成表面的吸附或固定是植入材料生物相容性的一个关键问题，特别是那些用于心脏手术需求的材料，也用于构建生物传感器或用作药物载体的纳米材料。研究的主题是分析两种类型的纤维状碳纳米材料的拉曼光谱，这两种材料在生物医学应用方面具有很大的潜力，它们与人血清白蛋白（HSA）孵育。第一种纳米材料是在通过电泳沉积（EPD）方法沉积的 MWCNT 层上制造的，然后通过化学气相沉积（CVD）过程引入的热解碳薄膜覆盖。第二种材料是由聚丙烯腈（PAN）前体通过静电纺丝（ESCNFs）制备的碳化纳米纤维形成的，然后用热解碳（CVD）覆盖。对于两种研究表面，都观察到 G 带蓝移到约 1600cm 的位置，这清楚地表明了白蛋白（HSA）与表面的粘附。G 和 G'（2D）峰的位移用于评估碳纳米材料上的应力积累。表面纳米和微观形貌以及碳纳米材料的有序化方法对表面-蛋白质相互作用模式有重大影响。
