College of Animal Science, Inner Mongolia Agricultural University, Hohhot, 010018, China.
Cell Stress Chaperones. 2018 Jul;23(4):703-710. doi: 10.1007/s12192-018-0882-5. Epub 2018 Feb 17.
The protective effects of chitosan (CS) supplementations on oxidative stress induced by diquat in weaned piglets were investigated. A total of 36 crossbreed piglets with an average live body weight (BW) of 8.80 ± 0.53 kg were weaned at 28 ± 2 days and randomly divided into six dietary treatments (n = 6): control (basal diet), negative control (10 mg diquat/kg BW injected to piglets fed with basal diet), and basal diet treatments containing either 250, 500, 1000, or 2000 mg/kg of CS administered to piglets injected with 10 mg diquat/kg BW. The experiment conducted for 21 days which consisted of pre-starter period (14 days) and starter period (7 days). BW, feed intake, and fecal consistency were monitored. Blood samples were collected to determine antioxidative and immune parameters. CS supplementation improved the growth performance and decreased fecal score of piglets from days 1 to 14. Diquat also induced oxidative stress and inflammatory responses by decreasing the activities of antioxidant and regulating cytokines. But dietary CS alleviated these negative effects induced by diquat that showed decreasing serum concentrations of pro-inflammatory cytokines but increasing activities of antioxidant enzymes and anti-inflammatory cytokines. Results indicated that CS attenuated the oxidative stress of piglets caused by diquat injection.
本研究旨在探讨壳聚糖（CS）补充剂对二氯喹啉酸诱导的断奶仔猪氧化应激的保护作用。将 36 头平均体重（BW）为 8.80±0.53kg 的杂交仔猪于 28±2 日龄断奶，随机分为 6 个日粮处理组（n=6）：对照组（基础日粮）、阴性对照组（10mg 二氯喹啉酸/kg BW 注射至基础日粮喂养的仔猪）和基础日粮处理组，分别在基础日粮中添加 250、500、1000 或 2000mg/kg CS，然后给注射 10mg 二氯喹啉酸/kg BW 的仔猪进行处理。试验期为 21 天，包括预饲期（14 天）和育肥期（7 天）。监测 BW、采食量和粪便稠度。采集血样以确定抗氧化和免疫参数。CS 补充剂提高了仔猪从第 1 天到第 14 天的生长性能并降低了粪便评分。二氯喹啉酸还通过降低抗氧化剂活性和调节细胞因子来诱导氧化应激和炎症反应。但日粮 CS 缓解了二氯喹啉酸引起的这些负面效应，表现为降低血清促炎细胞因子浓度，但增加抗氧化酶和抗炎细胞因子活性。结果表明，CS 减轻了二氯喹啉酸注射引起的仔猪氧化应激。
