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叶片提取物的DNA损伤保护活性及抗氧化潜力

DNA Damage Protecting Activity and Antioxidant Potential of Leaves Extract.

作者信息

Adinortey Michael Buenor, Ansah Charles, Weremfo Alexander, Adinortey Cynthia Ayefoumi, Adukpo Genevieve Etornam, Ameyaw Elvis Ofori, Nyarko Alexander Kwadwo

机构信息

Department of Biochemistry, School of Biological Sciences, University of Cape Coast, Cape Coast, Ghana.

Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.

出版信息

J Nat Sci Biol Med. 2018 Jan-Jun;9(1):6-13. doi: 10.4103/jnsbm.JNSBM_22_17.

DOI:10.4103/jnsbm.JNSBM_22_17
PMID:29456385
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5812077/
Abstract

BACKGROUND

The leaf extract of commonly known as African Lettuce is used locally to treat dyslipidemia and liver diseases, which are associated with oxidative stress. Methanol extract from leaves was tested for its antioxidant activity and its ability to protect DNA from oxidative damage.

MATERIALS AND METHODS

antioxidant potential of the leaf extract was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH), nitric oxide (NO), and hydroxyl (OH) radical scavenging assays. Ferric reducing power, total antioxidant capacity (TAC), metal chelating, and anti-lipid peroxidation ability of the extract were also examined using gallic acid, ascorbic acid, citric acid, and ethylenediaminetetraacetic acid as standards.

RESULTS

leaves extract showed antioxidant activity with IC values of 16.18 μg/ml (DPPH), 123.3 μg/ml (NO), 128.2 μg/ml (OH radical), 97.94 μg/ml (metal chelating), 80.28 μg/ml (TAC), and 23 μg/ml (anti-lipid peroxidation activity). leaves extract exhibited a strong capability for DNA damage protection at 20 mg/ml concentration.

CONCLUSION

These findings suggest that the methanolic leaf extract of could be used as a natural antioxidant and also as a preventive therapy against diseases such as arteriosclerosis associated with DNA damage.

摘要

背景

俗称非洲生菜的植物叶提取物在当地用于治疗与氧化应激相关的血脂异常和肝脏疾病。对该植物叶的甲醇提取物进行了抗氧化活性及其保护DNA免受氧化损伤能力的测试。

材料与方法

使用1,1-二苯基-2-苦基肼（DPPH）、一氧化氮（NO）和羟自由基（OH）清除试验评估叶提取物的抗氧化潜力。还以没食子酸、抗坏血酸、柠檬酸和乙二胺四乙酸为标准，检测了提取物的铁还原能力、总抗氧化能力（TAC）、金属螯合能力和抗脂质过氧化能力。

结果

该植物叶提取物表现出抗氧化活性，其IC值分别为16.18μg/ml（DPPH）、123.3μg/ml（NO）、128.2μg/ml（OH自由基）、97.94μg/ml（金属螯合）、80.28μg/ml（TAC）和23μg/ml（抗脂质过氧化活性）。该植物叶提取物在浓度为20mg/ml时表现出很强的保护DNA免受损伤的能力。

结论

这些发现表明，该植物叶的甲醇提取物可作为天然抗氧化剂，也可作为针对与DNA损伤相关的疾病（如动脉硬化）的预防性治疗药物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f24/5812077/476b9a50d9cd/JNSBM-9-6-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f24/5812077/eed85d934188/JNSBM-9-6-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f24/5812077/f727d6b116f1/JNSBM-9-6-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f24/5812077/84cd545a8618/JNSBM-9-6-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f24/5812077/97b0aa4a799a/JNSBM-9-6-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f24/5812077/d1629b318a33/JNSBM-9-6-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f24/5812077/4cb4a2bf2597/JNSBM-9-6-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f24/5812077/24294507a77c/JNSBM-9-6-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f24/5812077/476b9a50d9cd/JNSBM-9-6-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f24/5812077/eed85d934188/JNSBM-9-6-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f24/5812077/f727d6b116f1/JNSBM-9-6-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f24/5812077/84cd545a8618/JNSBM-9-6-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f24/5812077/97b0aa4a799a/JNSBM-9-6-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f24/5812077/d1629b318a33/JNSBM-9-6-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f24/5812077/4cb4a2bf2597/JNSBM-9-6-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f24/5812077/24294507a77c/JNSBM-9-6-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f24/5812077/476b9a50d9cd/JNSBM-9-6-g010.jpg

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2
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OMICS. 2016 Sep;20(9):528-37. doi: 10.1089/omi.2016.0117.
3
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Sci Rep. 2021 May 20;11(1):10656. doi: 10.1038/s41598-021-88710-w.
4
The Ethnopharmacological and Nutraceutical Relevance of (Willd.) Amin ex C. Jeffrey.（威尔德）阿明经C.杰弗里鉴定的（植物）的民族药理学和营养保健学相关性
Evid Based Complement Alternat Med. 2018 Jul 25;2018:7259146. doi: 10.1155/2018/7259146. eCollection 2018.
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5
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Chem Cent J. 2012 Feb 6;6(1):12. doi: 10.1186/1752-153X-6-12.
7
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