Oliveira Maxwel C, Gaines Todd A, Jhala Amit J, Knezevic Stevan Z
Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Concord, NE, United States.
Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, CO, United States.
Front Plant Sci. 2018 Feb 2;9:60. doi: 10.3389/fpls.2018.00060. eCollection 2018.
A population of (var. ) evolved resistance to 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitor herbicides (mesotrione, tembotrione, and topramezone) in Nebraska. The level of resistance was the highest to mesotrione, and the mechanism of resistance in this population is metabolism-based likely via cytochrome P450 enzymes. The increasing number of weeds resistant to herbicides warrants studies on the ecology and evolutionary factors contributing for resistance evolution, including inheritance of resistance traits. In this study, we investigated the genetic control of mesotrione resistance in an population from Nebraska, USA. Results showed that reciprocal crosses in the F1 families exhibited nuclear inheritance, which allows pollen movement carrying herbicide resistance alleles. The mode of inheritance varied from incomplete recessive to incomplete dominance depending upon the F1 family. Observed segregation patterns for the majority of the F2 and back-cross susceptible (BC/S) families did not fit to a single major gene model. Therefore, multiple genes are likely to confer metabolism-based mesotrione resistance in this population from Nebraska. The results of this study aid to understand the genetics and inheritance of a non-target-site based mesotrione resistant population from Nebraska, USA.
内布拉斯加州的一个（变种）种群对4-羟基苯丙酮酸双加氧酶（HPPD）抑制剂除草剂（甲基磺草酮、苯唑草酮和环磺酮）产生了抗性。该种群对甲基磺草酮的抗性水平最高，其抗性机制可能是基于细胞色素P450酶的代谢作用。对除草剂产生抗性的杂草数量不断增加，这就需要对导致抗性进化的生态和进化因素进行研究，包括抗性性状的遗传。在本研究中，我们调查了来自美国内布拉斯加州的一个种群对甲基磺草酮抗性的遗传控制。结果表明，F1代家族中的正反交表现出核遗传，这使得携带除草剂抗性等位基因的花粉能够传播。遗传模式因F1代家族而异，从不完全隐性到不完全显性。大多数F2代和回交易感（BC/S）家族观察到的分离模式不符合单一主基因模型。因此，多个基因可能赋予了来自内布拉斯加州的这个种群基于代谢的甲基磺草酮抗性。本研究结果有助于了解来自美国内布拉斯加州的一个基于非靶标位点的甲基磺草酮抗性种群的遗传学和遗传方式。
