Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Concord, NE, USA.
Department of Bioagricultural Sciences and Pest Management, Colorado State University in Fort Collins, CO, USA.
Pest Manag Sci. 2018 Oct;74(10):2296-2305. doi: 10.1002/ps.4697. Epub 2017 Sep 27.
A population of Amaranthus tuberculatus (var. rudis) was confirmed resistant to 4-hydroxyphenylpyruvate dioxygenase (HPPD)-inhibitor herbicides (mesotrione, tembotrione, and topramezone) in a seed corn/soybean rotation in Nebraska. Further investigation confirmed a non-target-site resistance mechanism in this population. The main objective of this study was to explore the role of cytochrome P450 inhibitors in restoring the efficacy of HPPD-inhibitor herbicides on the HPPD-inhibitor resistant A. tuberculatus population from Nebraska, USA (HPPD-R).
Enhanced metabolism via cytochrome P450 enzymes is the mechanism of resistance in HPPD-R. Amitrole partially restored the activity of mesotrione, whereas malathion, amitrole, and piperonyl butoxide restored the activity of tembotrione and topramezone in HPPD-R. Although corn was injured through malathion followed by mesotrione application a week after treatment, the injury was transient, and the crop recovered.
The use of cytochrome P450 inhibitors with tembotrione may provide a new way of controlling HPPD-inhibitor resistant A. tuberculatus, but further research is needed to identify the cytochrome P450 candidate gene(s) conferring metabolism-based resistance. The results presented here aid to gain an insight into non-target-site resistance weed management strategies. © 2017 Society of Chemical Industry.
在内布拉斯加州的玉米/大豆轮作中，确认了一种糙果苋（var. rudis）种群对 4-羟基苯基丙酮酸双加氧酶（HPPD）抑制剂类除草剂（mesotrione、tembotrione 和 topramezone）具有抗性。进一步的研究证实了该种群存在非靶标位点抗性机制。本研究的主要目的是探索细胞色素 P450 抑制剂在恢复 HPPD 抑制剂类除草剂对来自美国内布拉斯加州（HPPD-R）的糙果苋抗药性种群的功效中的作用。
通过细胞色素 P450 酶增强代谢是 HPPD-R 抗性的机制。阿米唑部分恢复了 mesotrione 的活性，而马拉硫磷、阿米唑和增效醚恢复了 tembotrione 和 topramezone 在 HPPD-R 中的活性。尽管马拉硫磷处理后一周接着施用 mesotrione 会导致玉米受损，但这种损伤是短暂的，作物会恢复。
与 tembotrione 一起使用细胞色素 P450 抑制剂可能为控制 HPPD 抑制剂抗性糙果苋提供一种新方法，但需要进一步研究以确定赋予代谢抗性的细胞色素 P450 候选基因。本文的结果有助于深入了解非靶标位点抗性杂草管理策略。 © 2017 英国化学学会。
