Thuaksuban Nuttawut, Monmaturapoj Naruporn, Luntheng Thunmaruk
Faculty of Dentistry, Department of Oral and Maxillofacial Surgery, Prince of Songkla University, Hatyai, Songkhla, Thailand.
National Metal and Materials Technology Center, Thailand Science Park, Pathumthani, Thailand.
Biomed Mater Eng. 2018;29(2):159-176. doi: 10.3233/BME-171720.
Polycaprolactone (PCL)-biphasic calcium phosphate (BCP) scaffolds fabricated using Melt-Stretching and Compression Molding (MSCM) can release calcium and phosphate ions, which are essential for bone formation.
Responses of the osteoblasts seeded on three groups of scaffolds including group A; PCL-20% BCP (%wt), group B; PCL-30% BCP and group C (control); pure PCL (100% PCL) were evaluated.
The cell-scaffold constructs were made by seeding osteoblast cell lines at 1×105 cells/scaffold. The constructs of each group were divided for culturing in proliferation medium (PR) and osteogenic induction medium (OS) for 30 days.
The cells attached and grew on the scaffolds of all groups. The cell number and the differentiation markers of groups A and B were remarkably higher than those of group C over the observation periods. Slow proliferation of the cells of group A and B in the PR medium in the first 7 days corresponded to the maximum increases in alkaline phosphatase activities (ALP). The maximum levels of ALP of those groups in the OS medium were not detected until day 14. The levels of osteocalcin of those groups were not statistically different when cultured in both mediums.
The MSCM scaffolds are suitable for supporting attachment and growth of the osteoblasts. Additional BCP into the PCL-based scaffolds accelerate early differentiation of the cells in the constructs even without osteogenic-inductive condition.
采用熔融拉伸与压缩成型（MSCM）制备的聚己内酯（PCL）-双相磷酸钙（BCP）支架可释放钙和磷酸根离子，这对骨形成至关重要。
评估接种于三组支架上的成骨细胞的反应，这三组支架包括A组：PCL-20% BCP（重量百分比）、B组：PCL-30% BCP和C组（对照组）：纯PCL（100% PCL）。
通过以1×105个细胞/支架接种成骨细胞系来制备细胞-支架构建体。将每组构建体分开，在增殖培养基（PR）和成骨诱导培养基（OS）中培养30天。
所有组支架上的细胞均能附着并生长。在观察期内，A组和B组的细胞数量及分化标志物显著高于C组。A组和B组细胞在PR培养基中最初7天增殖缓慢，这与碱性磷酸酶活性（ALP）的最大增加相对应。直到第14天，这些组在OS培养基中的ALP最高水平才被检测到。当在两种培养基中培养时，这些组的骨钙素水平无统计学差异。
MSCM支架适合支持成骨细胞的附着和生长。在基于PCL的支架中添加BCP即使在没有成骨诱导条件下也能加速构建体中细胞的早期分化。
