一种用作低剂量骨形态发生蛋白-2载体的新型聚己内酯-羟基磷灰石支架的生物活性：一项体外研究。
Bioactivity of a Novel Polycaprolactone-Hydroxyapatite Scaffold Used as a Carrier of Low Dose BMP-2: An In Vitro Study.
作者信息
Rittipakorn Pawornwan, Thuaksuban Nuttawut, Mai-Ngam Katanchalee, Charoenla Satrawut, Noppakunmongkolchai Warobon
机构信息
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Prince of Songkla University, Hatyai, Songkhla 90110, Thailand.
Ministry of Higher Education Science Research and Innovation (MHESRI), Ratchathewi, Bangkok 10400, Thailand.
出版信息
Polymers (Basel). 2021 Feb 1;13(3):466. doi: 10.3390/polym13030466.
Scaffolds of polycaprolactone-30% hydroxyapatite (PCL-30% HA) were fabricated using melt stretching and multilayer deposition (MSMD), and the in vitro response of osteoblasts to the scaffolds was assessed. In group A, the scaffolds were immersed in 10 µg/mL bone morphogenetic protein-2 (BMP-2) solution prior to being seeded with osteoblasts, and they were cultured in the medium without BMP-2. In group B, the cell-scaffold constructs without BMP-2 were cultured in medium containing 10 µg/mL BMP-2. The results showed greater cell proliferation in group A. The upregulation of runt-related transcription factor 2 and osteocalcin genes correlated with the release of BMP-2 from the scaffolds. The PCL-30% HA MSMD scaffolds appear to be suitable for use as osteoconductive frameworks and BMP-2 carriers.
采用熔融拉伸和多层沉积（MSMD）法制备了聚己内酯-30%羟基磷灰石（PCL-30% HA）支架，并评估了成骨细胞对该支架的体外反应。在A组中，将支架浸入10μg/mL骨形态发生蛋白-2（BMP-2）溶液中，然后接种成骨细胞，并在不含BMP-2的培养基中培养。在B组中，不含BMP-2的细胞-支架构建体在含有10μg/mL BMP-2的培养基中培养。结果显示A组细胞增殖更多。 runt相关转录因子2和骨钙素基因的上调与支架中BMP-2的释放相关。PCL-30% HA MSMD支架似乎适合用作骨传导框架和BMP-2载体。
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