Thuaksuban Nuttawut, Pannak Rungrot, Boonyaphiphat Pleumjit, Monmaturapoj Naruporn
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Prince of Songkla University, Hatyai, Songkhla 90112, Thailand.
Department of Pathology, Faculty of Medicine, Prince of Songkla University, Hatyai, Songkhla 90112, Thailand.
Biomed Mater Eng. 2018;29(2):253-267. doi: 10.3233/BME-171727.
Biocompatibility and degradation of poly ε-caprolactone (PCL)-Biphasic Calcium Phosphate (BCP) scaffolds fabricated by the "Melt Stretching and Compression Molding (MSCM)" technique were evaluated in rat models.
Degradation behaviors and histological biocompatibility of the PCL-20% BCP MSCM scaffolds and compare with those of PCL-20% β-tricalcium phosphate (TCP) scaffolds commercially fabricated by Fused Deposition Modeling (FDM) were evaluated.
The study groups included Group A: PCL-20% BCP MSCM scaffolds and Group B: PCL-20% TCP FDM scaffolds, which were implanted subcutaneously in twelve male Wistar rats. On day 14, 30, 60 and 90, dimensional changes of the scaffolds and their surrounding histological features were assessed using Micro-Computed Tomography (μ-CT) and histological analysis. Changes of their molecular weight were assessed using Gel Permeation Chromatography (GPC).
Formation of collagen and new blood vessels throughout the scaffolds of both groups increased with time with low degrees of inflammation. The μ-CT and GPC analysis demonstrated that the scaffolds of both groups degraded with time, but, their molecular weight slightly changed over the observation periods. All results of both groups were not significantly different.
The PCL-20% BCP MSCM scaffolds were biocompatible and biodegradable in vivo. Their properties were comparable to those of the commercial PCL-20% TCP scaffolds.
在大鼠模型中评估了通过“熔融拉伸与压缩成型（MSCM）”技术制备的聚ε-己内酯（PCL）-双相磷酸钙（BCP）支架的生物相容性和降解情况。
评估PCL-20% BCP MSCM支架的降解行为和组织学生物相容性，并与通过熔融沉积成型（FDM）商业制备的PCL-20%β-磷酸三钙（TCP）支架进行比较。
研究组包括A组：PCL-20% BCP MSCM支架和B组：PCL-20% TCP FDM支架，将其皮下植入12只雄性Wistar大鼠体内。在第14、30、60和90天，使用微型计算机断层扫描（μ-CT）和组织学分析评估支架的尺寸变化及其周围的组织学特征。使用凝胶渗透色谱法（GPC）评估其分子量变化。
两组支架内胶原蛋白和新血管的形成均随时间增加，炎症程度较低。μ-CT和GPC分析表明，两组支架均随时间降解，但其分子量在观察期内略有变化。两组的所有结果均无显著差异。
PCL-20% BCP MSCM支架在体内具有生物相容性和可生物降解性。其性能与商业PCL-20% TCP支架相当。
