Ding Songtao, Huang Wei, Qin Qizhong, Tang Jun, Liu Handeng
1 Department of Cell Biology and Genetics, Experimental Teaching Center, Chongqing Medical University, No. 1, Medical College Road, Yuzhong District, Chongqing 400016, People's Republic of China.
2 Chongqing Center for Disease Control and Prevention, Chongqing, 400042, People's Republic of China.
J Parasitol. 2018 Jun;104(3):297-301. doi: 10.1645/17-108. Epub 2018 Mar 9.
Among approximately 14 human-pathogenic microsporidian species, Enterocytozoon bieneusi is the most common. It can inhabit the small intestines, causing chronic diarrhea and wasting syndrome. Prevalence and genotype data for E. bieneusi in humans is available for only a few provinces of China. In the current study, 93 fecal specimens were collected from diarrheic children in Chongqing. Polymerase chain reaction amplification and sequencing of the internal transcribed spacer ( ITS) region of the E. bieneusi rDNA sequence identified 11 (11.83%) positive specimens. Among them, 8 (8.60%) are from patients of ages ranging from 2 mo to 6 yr old and 3 (3.23%) from patients 7 to 11 yr old. In total, 6 genotypes (4 novel genotypes and 2 known genotypes) were identified in this study. Phylogenetic analysis showed that all the genotypes identified in the present study belong to group 1, which previously has been described as a zoonotic group. This could mean these infections were acquired zoonotically, and it may be prudent to warn those people having close contact with animals of this potential risk.
在大约14种人类致病性微孢子虫物种中，比氏肠微孢子虫最为常见。它可寄生于小肠，导致慢性腹泻和消瘦综合征。目前中国仅有少数几个省份有关于人体中比氏肠微孢子虫的流行率和基因型数据。在本研究中，从重庆腹泻儿童中收集了93份粪便标本。对比氏肠微孢子虫核糖体DNA序列的内转录间隔区（ITS）进行聚合酶链反应扩增和测序，鉴定出11份（11.83%）阳性标本。其中，8份（8.60%）来自2个月至6岁的患者，3份（3.23%）来自7至11岁的患者。本研究共鉴定出6种基因型（4种新基因型和2种已知基因型）。系统发育分析表明，本研究中鉴定出的所有基因型均属于第1组，该组先前被描述为人畜共患病组。这可能意味着这些感染是通过人畜共患病途径获得的，因此向那些与动物密切接触的人警示这种潜在风险可能是谨慎的做法。
