Department of Oncology, The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, People's Republic of China.
Department of Oncology, The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, People's Republic of China.
Biochem Biophys Res Commun. 2018 Mar 4;497(2):694-699. doi: 10.1016/j.bbrc.2018.02.134. Epub 2018 Feb 16.
Fascin-1 is a cytoskeletal protein and it can specifically bind to F-actin, it can be abnormally expressed in a variety of solid tumors. Fascin-1 was identified as a factor for poor prognosis in non-small cell lung cancer (NSCLC). However, the relevant molecular mechanisms are not yet fully understood. In this study, the fascin-1 knockdown cells were produced by lentivirus infection, and then cell proliferation, invasion and cell migration assay were used to investigate the role of fascin-1 in NSCLC cells. The MAPK pathway related proteins were determined by western blot. In the current study, lentivirus-mediated fascin-1 knockdown significantly decreased the proliferation of NSCLC cells. Furthermore, fascin-1 silencing partly inhibited cell invasion and migration. Inhibition of fascin-1 decreased the activity of the MAPK pathway. Therefore, targeting fascin-1 may inhibit the growth and metastasis of NSCLC cells, which is a potentially effective therapeutic strategy for treating NSCLC.
Fascin-1 是一种细胞骨架蛋白，它可以特异性地结合 F-肌动蛋白，在多种实体瘤中异常表达。Fascin-1 被鉴定为非小细胞肺癌 (NSCLC) 预后不良的因素。然而，相关的分子机制尚不完全清楚。在这项研究中，通过慢病毒感染产生 fascin-1 敲低细胞，然后通过细胞增殖、侵袭和细胞迁移实验来研究 fascin-1 在 NSCLC 细胞中的作用。通过 Western blot 测定 MAPK 通路相关蛋白。在本研究中，慢病毒介导的 fascin-1 敲低显著降低了 NSCLC 细胞的增殖。此外，fascin-1 沉默部分抑制了细胞侵袭和迁移。抑制 fascin-1 降低了 MAPK 通路的活性。因此，靶向 fascin-1 可能抑制 NSCLC 细胞的生长和转移，这是治疗 NSCLC 的一种潜在有效治疗策略。
