Mohr Kathrin I, Moradi Azam, Glaeser Stefanie P, Kämpfer Peter, Gemperlein Katja, Nübel Ulrich, Schumann Peter, Müller Rolf, Wink Joachim
Microbial Drugs, Helmholtz Centre for Infection Research (HZI), 38124 Braunschweig, Germany.
Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran.
Int J Syst Evol Microbiol. 2018 Mar;68(3):721-729. doi: 10.1099/ijsem.0.002569. Epub 2018 Jan 18.
An orange-coloured myxobacterium, MNa11734, was isolated from desert in Iran. MNa11734 had rod-shaped vegetative cells, moved by gliding and was bacteriolytic. No real fruiting body formation could be observed, but sporangioles were produced on water agar. The strain was mesophilic, strictly aerobic and chemoheterotrophic. 16S rRNA gene analyses revealed that MNa11734 belonged to the family Nannocystaceae, genus Nannocystis and was closely related to Nannocystis pusilla Na p29 (DSM 14622) and Nannocystis exedens Na e1 (DSM 71), with 97.8 and 97.6 % 16S rRNA gene sequence similarity, respectively. Laboratory-measured DNA-DNA hybridization showed only 9.5/15.7 % (reciprocal) similarity between the novel strain and N. pusilla Na p29, and 14.1/20.4 % between the strain and N. exedens Na e1, whereas DNA-DNA hybridization estimates derived from draft genome sequences were 21.8-23.0 % and 22.2-23.7 %, respectively, depending on the calculation method. The G+C content of DNA from Nannocystis konarekensis MNa11734 was 73.3 mol%, for N. pusilla Nap29 it was 71.8 mol% and for N. exedens Nae1 it was 72.2 mol%. The major fatty acids of the new strain were C16 : 1 (56.2 %), iso-C17 : 0 (14.4 %), C14 : 0 (8.2 %), C16 : 0 (6.6 %) and iso-C15 : 0 (5.9 %). Strain MNa11734 exhibited phylogenetic and physiological similarities to the two other species of Nannocystis, i.e. N. pusilla and N. exedens, but the differences were sufficient enough to represent a novel species, for which the name Nannocystiskonarekensis sp. nov. is proposed. The type strain is MNa11734 (=DSM 104509=NCCB 100618).
从伊朗沙漠中分离出一种橙色粘细菌，命名为MNa11734。MNa11734具有杆状营养细胞，通过滑行运动，具有溶菌性。未观察到真正的子实体形成，但在水琼脂上产生了小孢子囊。该菌株为嗜温菌，严格需氧，属于化能异养型。16S rRNA基因分析表明，MNa11734属于囊球菌科、囊球菌属，与微小囊球菌Na p29（DSM 14622）和扩展囊球菌Na e1（DSM 71）密切相关，16S rRNA基因序列相似性分别为97.8%和97.6%。实验室测定的DNA-DNA杂交结果显示，该新菌株与微小囊球菌Na p29之间的相似性仅为9.5/15.7%（相互），与扩展囊球菌Na e1之间的相似性为14.1/20.4%，而根据基因组草图序列得出的DNA-DNA杂交估计值分别为21.8-23.0%和22.2-23.7%，具体取决于计算方法。康氏囊球菌MNa11734的DNA的G+C含量为73.3 mol%，微小囊球菌Nap29为有71.8 mol%，扩展囊球菌Nae1为72.2 mol%。新菌株的主要脂肪酸为C16 : 1（56.2%）、异-C17 : 0（14.4%）、C14 : 0（8.2%）、C16 : 0（6.6%）和异-C15 : 0（5.9%）。菌株MNa11734在系统发育和生理方面与囊球菌属的其他两个物种，即微小囊球菌和扩展囊球菌相似，但差异足以代表一个新物种，为此提出了新物种康氏囊球菌（Nannocystiskonarekensis sp. nov.）的名称。模式菌株为MNa11734（=DSM 104509=NCCB 100618）。
