Fang Chen, Wu Yue-Hong, Xamxidin Maripat, Wang Chun-Sheng, Xu Xue-Wei
Key Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China.
Int J Syst Evol Microbiol. 2017 Dec;67(12):5261-5267. doi: 10.1099/ijsem.0.002458. Epub 2017 Oct 31.
A Gram-stain-negative, aerobic, non-motile and rod-shaped bacterium, designated B1, was isolated from deep-sea sediment collected from the South Atlantic Ocean. Cells of strain B1 grew in medium containing 0.5-6.0 % (w/v) NaCl (optimum 1.0-2.0 %), at pH 5.5-7.5 (optimum pH 7.0) and at 15-37 °C (optimum 30 °C). Aesculin, starch and Tweens 20, 40 and 60 were hydrolysed. The strain was able to grow in medium containing a high concentration of Co (10 mM). The 16S rRNA gene sequence of strain B1 shared high similarity with respect to Maribacter orientalis DSM 16471 (97.7 %), and exhibited less than 97.0 % sequence similarity with the type strains of other species with validly published names. Phylogenetic analyses revealed that strain B1 fell within the cluster comprising Maribacter species and formed an independent lineage. The average nucleotide identity and in silico DNA-DNA hybridization values between strain B1 and M. orientalis DSM 16471 were 72.0 % and 17.6 %, respectively. Strain B1 contained menaquinone 6 (MK-6) as the sole isoprenoid quinone and iso-C17 : 0 3-OH, iso-C15 : 0 and iso-C15 : 1 G as the predominant cellular fatty acids. The major polar lipids were phosphatidylethanolamine, one unidentified aminolipid and two unidentified lipids. The DNA G+C content was 39.7 mol%. According to the phylogenetic, chemotaxonomic and phenotypic data, B1 represents a novel species of the genus Maribacter, for which the name Maribacter cobaltidurans sp. nov. is proposed. The type strain is B1 (=CGMCC 1.15508=KCTC 52882=MCCC 1K03318).
从南大西洋采集的深海沉积物中分离出一株革兰氏阴性、需氧、无运动性的杆状细菌，命名为B1。B1菌株的细胞能在含0.5 - 6.0%（w/v）NaCl（最适浓度为1.0 - 2.0%）、pH 5.5 - 7.5（最适pH 7.0）和15 - 37℃（最适30℃）的培养基中生长。七叶苷、淀粉以及吐温20、40和60能被水解。该菌株能够在含高浓度Co（10 mM）的培养基中生长。B1菌株的16S rRNA基因序列与东方海杆菌DSM 16471具有高度相似性（97.7%），与其他有效发表名称的种的模式菌株的序列相似性低于97.0%。系统发育分析表明，B1菌株属于海杆菌属的聚类群，并形成一个独立的分支。B1菌株与东方海杆菌DSM 16471之间的平均核苷酸同一性和电子计算机DNA - DNA杂交值分别为72.0%和17.6%。B1菌株含有甲基萘醌6（MK - 6）作为唯一的类异戊二烯醌，以及iso - C17 : 0 3 - OH、iso - C15 : 0和iso - C15 : 1 G作为主要的细胞脂肪酸。主要极性脂为磷脂酰乙醇胺、一种未鉴定的氨基脂和两种未鉴定的脂类。DNA G + C含量为39.7 mol%。根据系统发育、化学分类和表型数据，B1代表海杆菌属的一个新种，为此提出新名称钴耐受海杆菌（Maribacter cobaltidurans sp. nov.）。模式菌株为B1（=CGMCC 1.15508 = KCTC 52882 = MCCC 1K03318）。
