Choi Jun Young, Kim Jin Ho, Lee Pyung Cheon
Department of Molecular Science and Technology and Department of Applied Chemistry and Biological Engineering, Ajou University, Woncheon-dong, Yeongtong-gu, Suwon 16499, Republic of Korea.
Int J Syst Evol Microbiol. 2018 Mar;68(3):911-916. doi: 10.1099/ijsem.0.002610. Epub 2018 Feb 2.
Taxonomic studies were carried out on a carotenoid-producing strain, designated WV39, isolated from the faeces of Antarctic penguins. Cells of strain WV39 were Gram-stain-negative, strictly aerobic, yellow and rod-shaped. 16S rRNA gene sequence analysis revealed that strain WV39 was closely related to Flavobacterium qiangtangense JCM 19739 (96.3 % similarity), Flavobacterium noncentrifugens NBRC 108844 (95.5 %) and Flavobacterium aquatile LMG 4008 (94.9 %). The predominant cellular fatty acids were iso-C15 : 0, iso-C15 : 0 3-OH and summed feature 3 (comprising iso-C15 : 0 2-OH and/or C16 : 1ω7c). Menaquinone-6 was the sole quinone identified, and the major pigment was zeaxanthin. The major polar lipid was phosphatidylethanolamine. DNA-DNA relatedness of strain WV39 with respect to its closest phylogenetic neighbours was 41.8 % for F. qiangtangense JCM 19739, 25.5 % for F. aquatile LMG 4008 and 25.2 % for F. noncentrifugens NBRC 108844. The DNA G+C content of strain WV39 was 39.8 mol%. Based on the phenotypic, chemotaxonomic and phylogenetic data, strain WV39 is concluded to represent a novel species of the genus Flavobacterium, for which the name Flavobacteriumkingsejongi sp. nov. is proposed. The type strain is WV39 (=KCTC 42908=CECT 9085).
对从南极企鹅粪便中分离出的一株产类胡萝卜素菌株WV39进行了分类学研究。WV39菌株的细胞革兰氏染色阴性，严格需氧，呈黄色，杆状。16S rRNA基因序列分析表明，WV39菌株与强塘黄杆菌JCM 19739（相似度96.3%）、非离心黄杆菌NBRC 108844（95.5%）和水生黄杆菌LMG 4008（94.9%）密切相关。主要的细胞脂肪酸为异C15:0、异C15:0 3-OH和特征性总和3（包括异C15:0 2-OH和/或C16:1ω7c）。鉴定出的唯一醌类为甲基萘醌-6，主要色素为玉米黄质。主要极性脂为磷脂酰乙醇胺。WV39菌株与其最接近的系统发育邻居的DNA-DNA相关性，与强塘黄杆菌JCM 19739为41.8%，与水生黄杆菌LMG 4008为25.5%，与非离心黄杆菌NBRC 108844为25.2%。WV39菌株的DNA G+C含量为39.8 mol%。基于表型、化学分类和系统发育数据，得出WV39菌株代表黄杆菌属一个新物种的结论，为此提出新名称金世宗黄杆菌（Flavobacterium kingsejongi sp. nov.）。模式菌株为WV39（=KCTC 42908=CECT 9085）。
