Gan Longzhan, Long Xiufeng, Zhang Heming, Hou Yanyan, Tian Jiewei, Zhang Yuqin, Tian Yongqiang
Key Laboratory of Leather Chemistry and Engineering, Ministry of Education and College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu 610065, PR China.
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and PeKing Union Medical College, Beijing 100050, PR China.
Int J Syst Evol Microbiol. 2018 Apr;68(4):1153-1159. doi: 10.1099/ijsem.0.002644. Epub 2018 Feb 19.
A novel, Gram-stain-negative, aerobic, rod-shaped, motile and moderately halophilic bacterium, designated strain LCB169, was isolated from a saline soil sample from Gansu Province, PR China. The cells of LCB169 grew at 10-52 °C (optimum 30 °C), at pH 6.0-10.0 (optimum pH 8.0) and in the presence of 0-17 % (w/v) NaCl (optimum 10-15 %). Phylogenetic analysis based on 16S rRNA gene sequences and concatenated 16S rRNA, gyrB and rpoD genes sequences revealed that LCB169 represented a member of the genus Halomonas in the class Gammaproteobacteria. The most closely related species were Halomonas daqingensis DQD2-30 (98.0 % 16S rRNA gene sequence similarity), Halomonas kenyensis AIR-2 (97.8 %) and Halomonas desiderata FB2 (97.5 %). DNA-DNA relatedness values between LCB169 and H. daqingensis CGMCC 1.6443, H. desiderata DSM 9502 and H. kenyensis DSM 17331 were 33, 35 and 38 %, respectively. The polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and three unidentified phospholipids. The major fatty acids were C18 : 1ω7c, C16 : 0, C16 : 1ω7c and C12 : 0 3-OH. The genomic DNA G+C content was 66.1 mol% and the predominant respiratory quinone was Q-9. On the basis of the results of phenotypic, phylogenetic and chemotaxonomic analyses and DNA-DNA hybridization relatedness values, LCB169 is considered to represent a novel species of the genus Halomonas, for which the name Halomonas saliphila sp. nov. is proposed. The type strain is LCB169 (=CGMCC 1.15818=KCTC 52618).
从中国甘肃省的一份盐土样本中分离出了一株新型革兰氏阴性、需氧、杆状、具运动性且中度嗜盐的细菌，命名为菌株LCB169。LCB169的细胞在10 - 52 °C（最适温度30 °C）、pH 6.0 - 10.0（最适pH 8.0）以及0 - 17 %（w/v）NaCl（最适浓度10 - 15 %）的条件下生长。基于16S rRNA基因序列以及串联的16S rRNA、gyrB和rpoD基因序列的系统发育分析表明，LCB169代表γ-变形菌纲盐单胞菌属的一个成员。与其亲缘关系最近的物种是大庆盐单胞菌DQD2 - 30（16S rRNA基因序列相似性为98.0 %）、肯尼亚盐单胞菌AIR - 2（97.8 %）和所需盐单胞菌FB2（97.5 %）。LCB169与大庆盐单胞菌CGMCC 1.6443、所需盐单胞菌DSM 9502和肯尼亚盐单胞菌DSM 17331之间的DNA - DNA杂交相关值分别为33%、35%和38 %。极性脂被鉴定为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱以及三种未鉴定的磷脂。主要脂肪酸为C18 : 1ω7c、C16 : 0、C16 : 1ω7c和C12 : 0 3 - OH。基因组DNA的G + C含量为66.1 mol%，主要呼吸醌为Q - 9。基于表型、系统发育和化学分类分析结果以及DNA - DNA杂交相关值，LCB169被认为代表盐单胞菌属的一个新物种，为此提出新名称嗜盐盐单胞菌（Halomonas saliphila sp. nov.）。模式菌株为LCB169（=CGMCC 1.15818 = KCTC 52618）。
