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sp. nov.,一种从中性盐腌皮中分离出来的中度嗜盐细菌。

sp. nov., a moderately halophilic bacterium isolated from wetsalted hides.

机构信息

Key Laboratory of Leather Chemistry and Engineering (Sichuan University), Ministry of Education, Chengdu 610065, PR China.

College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, PR China.

出版信息

Int J Syst Evol Microbiol. 2020 Oct;70(10):5417-5424. doi: 10.1099/ijsem.0.004426. Epub 2020 Sep 4.

Abstract

A Gram-stain-negative, moderately halophilic strain, designated strain L5, was isolated from wetsalted hides collected from Chengdu, south-west PR China. The cells were motile, facultative aerobic, short rod-shaped and non-endospore-forming. Growth of strain L5 occurred at pH 6-10 (optimum, pH 8), 10-45 °C (optimum, 30 °C) and in the presence of 1-17 % (w/v) NaCl (optimum, 10 %). Results of phylogenetic analyses based on 16S rRNA, and gene sequences and its genome revealed that strain L5 belonged to the genus . Strain L5 was found to be most closely related to the type strains of , , , and (98.8, 98.6, 98.3, 97.9 and 97.4 % 16S rRNA gene sequence similarity, respectively). The draft genome was approximately 4.2 Mb in size with a G+C content of 63.5 mol%. The average nucleotide identity (ANI) and digital DNA-DNA hybridization values among strain L5 and the selected species were 83.3-88.9 % (ANIm), 71.1-87.3 % (ANIb) and 20.2-34.6 %, which are below the recommended cutoff values. Major fatty acids were C, C 7, C 7 and C cyclo 8 and the predominant ubiquinone was Q-9, with minor ubiquinone Q-8 also present. The phospholipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, four unidentified aminophospholipids and three unidentified phospholipids. Based on the mentioned polyphasic taxonomic evidence, strain L5 represents a novel species within the genus , for which sp. nov. is proposed. The type strain is L5 (=CGMCC 1.17335=KCTC 72573).

摘要

一株革兰氏阴性、中度嗜盐的菌株,命名为 L5 株,从中国西南部成都采集的湿腌皮中分离得到。细胞呈运动性、兼性需氧、短杆状、非芽孢形成。L5 株的生长条件为 pH6-10(最佳 pH8)、10-45°C(最佳 30°C)和 1-17%(w/v)NaCl(最佳 10%)。基于 16S rRNA 基因和基因组序列的系统发育分析结果表明,该菌株属于 属。L5 株与 属的模式菌株 、 、 、 和 (分别为 98.8%、98.6%、98.3%、97.9%和 97.4%的 16S rRNA 基因序列相似性)最为密切相关。该菌株的基因组草图大小约为 4.2Mb,G+C 含量为 63.5mol%。L5 株与选定的 种之间的平均核苷酸同一性(ANI)和数字 DNA-DNA 杂交值分别为 83.3-88.9%(ANIm)、71.1-87.3%(ANIb)和 20.2-34.6%,均低于推荐的临界值。主要脂肪酸为 C、C 7、C 7 和 C 环 8,主要醌类为 Q-9,也存在少量的醌类 Q-8。磷脂谱由双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱、四种未鉴定的氨基磷脂和三种未鉴定的磷脂组成。基于上述多相分类学证据,L5 株代表了 属中的一个新种,建议命名为 sp. nov.。模式菌株为 L5(=CGMCC 1.17335=KCTC 72573)。

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