Key Laboratory of Leather Chemistry and Engineering (Sichuan University), Ministry of Education, Chengdu 610065, PR China.
College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, PR China.
Int J Syst Evol Microbiol. 2020 Oct;70(10):5417-5424. doi: 10.1099/ijsem.0.004426. Epub 2020 Sep 4.
A Gram-stain-negative, moderately halophilic strain, designated strain L5, was isolated from wetsalted hides collected from Chengdu, south-west PR China. The cells were motile, facultative aerobic, short rod-shaped and non-endospore-forming. Growth of strain L5 occurred at pH 6-10 (optimum, pH 8), 10-45 °C (optimum, 30 °C) and in the presence of 1-17 % (w/v) NaCl (optimum, 10 %). Results of phylogenetic analyses based on 16S rRNA, and gene sequences and its genome revealed that strain L5 belonged to the genus . Strain L5 was found to be most closely related to the type strains of , , , and (98.8, 98.6, 98.3, 97.9 and 97.4 % 16S rRNA gene sequence similarity, respectively). The draft genome was approximately 4.2 Mb in size with a G+C content of 63.5 mol%. The average nucleotide identity (ANI) and digital DNA-DNA hybridization values among strain L5 and the selected species were 83.3-88.9 % (ANIm), 71.1-87.3 % (ANIb) and 20.2-34.6 %, which are below the recommended cutoff values. Major fatty acids were C, C 7, C 7 and C cyclo 8 and the predominant ubiquinone was Q-9, with minor ubiquinone Q-8 also present. The phospholipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, four unidentified aminophospholipids and three unidentified phospholipids. Based on the mentioned polyphasic taxonomic evidence, strain L5 represents a novel species within the genus , for which sp. nov. is proposed. The type strain is L5 (=CGMCC 1.17335=KCTC 72573).
一株革兰氏阴性、中度嗜盐的菌株，命名为 L5 株，从中国西南部成都采集的湿腌皮中分离得到。细胞呈运动性、兼性需氧、短杆状、非芽孢形成。L5 株的生长条件为 pH6-10（最佳 pH8）、10-45°C（最佳 30°C）和 1-17%（w/v）NaCl（最佳 10%）。基于 16S rRNA 基因和基因组序列的系统发育分析结果表明，该菌株属于 属。L5 株与 属的模式菌株 、 、 、 和 （分别为 98.8%、98.6%、98.3%、97.9%和 97.4%的 16S rRNA 基因序列相似性）最为密切相关。该菌株的基因组草图大小约为 4.2Mb，G+C 含量为 63.5mol%。L5 株与选定的 种之间的平均核苷酸同一性（ANI）和数字 DNA-DNA 杂交值分别为 83.3-88.9%（ANIm）、71.1-87.3%（ANIb）和 20.2-34.6%，均低于推荐的临界值。主要脂肪酸为 C、C 7、C 7 和 C 环 8，主要醌类为 Q-9，也存在少量的醌类 Q-8。磷脂谱由双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱、四种未鉴定的氨基磷脂和三种未鉴定的磷脂组成。基于上述多相分类学证据，L5 株代表了 属中的一个新种，建议命名为 sp. nov.。模式菌株为 L5（=CGMCC 1.17335=KCTC 72573）。
