Xiao Zijun, Shen Jie, Wang Zhuo, Dong Fanghui, Zhao Jing-Yi
Center for Bioengineering and Biotechnology, College of Chemical Engineering, China University of Petroleum (East China), Qingdao, 266580, China.
Curr Microbiol. 2021 Jan;78(1):397-402. doi: 10.1007/s00284-020-02268-w. Epub 2020 Nov 2.
A novel aerobic bacterium designated DX6 was isolated from a Gobi soil sample collected in Bachu County, China. Cells are Gram-stain-negative and rod-shaped and colonies are creamy, circular and smooth. The growth range of NaCl concentration was 1-15% (optimum 2-10%, w/v). Growth occurs at 10-45 °C (optimum 37 °C) and pH 5.0-10.0 (optimum pH 7.0-9.0). Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain DX6 formed a distinct lineage in the clade of genus Halomonas and is related to Halomonas desiderata DSM 9502 (98.3%), Halomonas kenyensis AIR-2 (97.7%), Halomonas daqingensis DQD2-30 (97.6%), Halomonas saliphila LCB169 (97.4%) and Halomonas endophytica MC28 (96.2%). Analysis of the housekeeping genes gryB and rpoD and calculation of the average nucleotide identities and the digital DNA-DNA hybridization values between strain DX6 and the related type Halomonas strains further revealed that strain DX6 represented a distinct species. The main respiratory quinones of strain DX6 were ubiquinone 9 (Q-9) and ubiquinone 8 (Q-8). The predominant cellular fatty acids were summed feature 8 (Cω7c and/or Cω6c), summed feature 3 (Cω7c and/or Cω6c) and C. The major polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, an unidentified phosphatidylglycolipid, and four unidentified lipids. Based on the phenotypic, phylogenetic, chemotaxonomic and genomic features, strain DX6 represents a novel species of the genus Halomonas. The name Halomonas bachuensis sp. nov. is proposed with strain DX6 (= CCTCC AB 2020094 = KCTC 82196) designated as the type strain.
从中国巴楚县采集的戈壁土壤样本中分离出一株名为DX6的新型需氧细菌。细胞革兰氏染色阴性，呈杆状，菌落呈奶油色、圆形且光滑。NaCl浓度的生长范围为1-15%（最适为2-10%，w/v）。在10-45°C（最适37°C）和pH 5.0-10.0（最适pH 7.0-9.0）条件下生长。对16S rRNA基因序列的系统发育分析表明，菌株DX6在嗜盐单胞菌属分支中形成了一个独特的谱系，与期望嗜盐单胞菌DSM 9502（98.3%）、肯尼亚嗜盐单胞菌AIR-2（97.7%）、大庆嗜盐单胞菌DQD2-30（97.6%）、嗜盐嗜盐单胞菌LCB169（97.4%）和内生嗜盐单胞菌MC28（96.2%）相关。对看家基因gryB和rpoD的分析以及菌株DX6与相关嗜盐单胞菌模式菌株之间平均核苷酸同一性和数字DNA-DNA杂交值的计算进一步表明，菌株DX6代表一个独特的物种。菌株DX6的主要呼吸醌为泛醌9（Q-9）和泛醌8（Q-8）。主要的细胞脂肪酸为总和特征8（Cω7c和/或Cω6c）、总和特征3（Cω7c和/或Cω6c）和C。主要极性脂质由双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、两种未鉴定的磷脂、一种未鉴定的磷脂糖脂和四种未鉴定的脂质组成。基于表型、系统发育、化学分类和基因组特征，菌株DX6代表嗜盐单胞菌属的一个新物种。提出新物种名称巴楚嗜盐单胞菌（Halomonas bachuensis sp. nov.），以菌株DX6（= CCTCC AB 2020094 = KCTC 82196）作为模式菌株。
