Department of Urology, the First Affiliated Hospital of Huzhou University, Huzhou, Zhejiang, China.
Eur Rev Med Pharmacol Sci. 2018 Feb;22(3):671-677. doi: 10.26355/eurrev_201802_14292.
Renal cell carcinoma (RCC) is the most common malignant tumor in the urogenital system. Its easily metastatic characteristics greatly reduce the postoperative survival rate. NudCD1, as a proto-oncogene, may be involved in the proliferation, migration, and invasion of renal cell carcinoma cell. This study intends to explore the expression of NudCD1 in renal cancer tissue and its effect on renal cell behavior.
NudCD1 expression in RCC tissue was tested Western blot. The cellular localization of NudCD1 was detected by immunohistochemistry (IHC). NudCD1 highly expressed RCC cell line was selected. NudCD1 knockdown or overexpression was performed through cell transfection. Cell proliferation, migration, and invasion were assessed by MTT assay, wound scratch assay, and transwell assay, respectively.
NudCD1 mainly located in the cytoplasm and significantly upregulated in RCC tissue compared with adjacent normal control (p < 0.05). NudCD1 expressed highest in A498 cell line among several RCC cell lines. NudCD1 expression was positively correlated with cell proliferation, migration, and invasion in A498. NudCD1 may be treated as a key factor in regulating cell behavior.
NudCD1 significantly increased in RCC and was positively correlated with cell proliferation, migration, and invasion. It could be used as an indicator for the early screening and potential treatment target for RCC.
肾细胞癌（RCC）是泌尿生殖系统最常见的恶性肿瘤。其易转移的特点极大地降低了术后的存活率。NudCD1 作为原癌基因，可能参与了肾癌细胞的增殖、迁移和侵袭。本研究旨在探讨 NudCD1 在肾癌组织中的表达及其对肾细胞行为的影响。
采用 Western blot 检测 RCC 组织中 NudCD1 的表达。采用免疫组织化学（IHC）检测 NudCD1 的细胞定位。选择 NudCD1 高表达的 RCC 细胞系。通过细胞转染实现 NudCD1 的敲低或过表达。通过 MTT 检测、划痕实验和 Transwell 检测分别评估细胞增殖、迁移和侵袭。
NudCD1 主要位于细胞质中，与相邻正常对照相比在 RCC 组织中显著上调（p<0.05）。在几种 RCC 细胞系中，NudCD1 在 A498 细胞系中表达最高。NudCD1 的表达与 A498 细胞的增殖、迁移和侵袭呈正相关。NudCD1 可能被视为调节细胞行为的关键因素。
NudCD1 在 RCC 中显著增加，并与细胞增殖、迁移和侵袭呈正相关。它可以作为 RCC 早期筛查的指标和潜在的治疗靶点。
