Avian Health Research Unit, Department of Veterinary Medicine, Faculty of Veterinary Science, Chulalongkorn University, Patumwan, Bangkok 10330, Thailand.
Poult Sci. 2018 Jun 1;97(6):1934-1940. doi: 10.3382/ps/pey051.
Mycoplasma gallisepticum (MG) causes respiratory signs and economic losses in the poultry industry. MG vaccination is one of the effective prevention and control measures that have been used around the world. Our previous study demonstrated that chitosan-adjuvanted MG bacterin could effectively reduce pathological lesions induced by MG and that chitosan could be used as an adjuvant in MG bacterin. The present study determining the efficacy of MG bacterins against the Thai MG strain was based on vaccine programs. Seven groups (25 layers/group) were received MG bacterins containing 0.5% chitosan or a commercial bacterin via intramuscular (IM) or intraocular (IO) route at 6 and 10 wk of age. Sham-negative and sham-positive controls were groups 1 and 2, respectively. Group 3: IM route of chitosan bacterin followed by IM route of chitosan bacterin; group 4: commercial bacterin via IM route followed by chitosan bacterin via IO route; group 5: commercial bacterin via IM route followed by commercial bacterin via IM route; group 6: chitosan bacterin via IM followed by chitosan bacterin via IO route; and group 7: chitosan bacterin via IO route followed by chitosan bacterin via IO route were determined. At 16 wk of age, all groups, excluding group 1, were challenged intratracheally with 0.1 mL containing Thai MG strain 107 colony-forming unit. At 17, 18, and 20 wk of age, 5 birds in each group were bled for serological testing and swabbed at the choanal cleft for the quantitative real-time PCR assay, the euthanized and necropsied. The results showed that birds vaccinated with a commercial intramuscular bacterin followed by an intraocularly chitosan adjuvant bacterin showed the best protection against the MG challenge. The study indicated that chitosan could be the effective mucosal adjuvant and increased the effectiveness of MG bacterin.
鸡毒支原体（MG）可引起家禽养殖业的呼吸道疾病和经济损失。MG 疫苗接种是世界范围内使用的有效预防和控制措施之一。我们之前的研究表明，壳聚糖佐剂 MG 菌苗可有效减轻 MG 引起的病理损伤，壳聚糖可用作 MG 菌苗的佐剂。本研究基于疫苗计划，确定了 MG 菌苗对泰国 MG 株的功效。7 组（每组 25 只）在 6 和 10 周龄时通过肌肉内（IM）或眼内（IO）途径接种含有 0.5%壳聚糖的 MG 菌苗或商业菌苗。阴性和阳性假手术对照组分别为第 1 组和第 2 组。第 3 组：壳聚糖菌苗的 IM 途径，然后是壳聚糖菌苗的 IM 途径；第 4 组：IM 途径接种商业菌苗，然后 IO 途径接种壳聚糖菌苗；第 5 组：IM 途径接种商业菌苗，然后 IM 途径接种商业菌苗；第 6 组：IM 途径接种壳聚糖菌苗，然后 IO 途径接种壳聚糖菌苗；第 7 组：IO 途径接种壳聚糖菌苗，然后 IO 途径接种壳聚糖菌苗。在 16 周龄时，除第 1 组外，所有组均通过气管内滴注 0.1 mL 含泰国 MG 株 107 个菌落形成单位的混合物进行攻毒。在 17、18 和 20 周龄时，每组 5 只鸡采血进行血清学检测，在鼻后孔拭子进行定量 real-time PCR 检测，然后安乐死并进行尸检。结果表明，肌肉内接种商业菌苗，然后眼内接种壳聚糖佐剂菌苗的鸡对 MG 攻毒的保护效果最好。研究表明，壳聚糖可以作为有效的黏膜佐剂，提高 MG 菌苗的效果。
