Zhang Min, Zhang Hongyuan, Tan Jie, Huang Shuping, Chen Xia, Jiang Daohong, Xiao Xueqiong
State Key Laboratory of Agricultural Microbiology, Hubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Wuhan Academy of Agricultural Sciences, Wuhan 430065, China.
Pathogens. 2021 Apr 13;10(4):470. doi: 10.3390/pathogens10040470.
Eggplant ( L.), which belongs to the Solanaceae family, is an important vegetable crop. However, its production is severely threatened by root-knot nematodes (RKNs) in many countries. , a wild relative of eggplant, is employed worldwide as rootstock for eggplant cultivation due to its resistance to soil-borne diseases such as RKNs. In this study, to identify the RKN defense mechanisms, the transcriptomic profiles of eggplant and were compared. A total of 5360 differentially expressed genes (DEGs) were identified for the response to RKN infection. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis showed that these DEGs are mainly involved in the processes of response to stimulus, protein phosphorylation, hormone signal transduction, and plant-pathogen interaction pathways. Many phytohormone-related genes and transcription factors (MYB, WRKY, and NAC) were differentially expressed at the four time points (ck, 7, 14, and 28 days post-infection). The abscisic acid signaling pathway might be involved in plant-nematode interactions. qRT-PCR validated the expression levels of some of the DEGs in eggplant. These findings demonstrate the nematode-induced expression profiles and provide some insights into the nematode resistance mechanism in eggplant.
茄子（Solanum melongena L.）属于茄科，是一种重要的蔬菜作物。然而，在许多国家，其生产受到根结线虫（RKNs）的严重威胁。托鲁巴姆（Solanum torvum）作为茄子的野生近缘种，因其对根结线虫等土传病害具有抗性，在全球范围内被用作茄子栽培的砧木。在本研究中，为了确定抗根结线虫的防御机制，比较了茄子和托鲁巴姆的转录组图谱。共鉴定出5360个响应根结线虫感染的差异表达基因（DEGs）。基因本体论和京都基因与基因组百科全书富集分析表明，这些差异表达基因主要参与对刺激的响应、蛋白质磷酸化、激素信号转导和植物-病原体相互作用途径。许多与植物激素相关的基因和转录因子（MYB、WRKY和NAC）在四个时间点（接种后0、7、14和28天）差异表达。脱落酸信号通路可能参与植物与线虫的相互作用。qRT-PCR验证了茄子中一些差异表达基因的表达水平。这些发现揭示了线虫诱导的表达谱，并为茄子的抗线虫机制提供了一些见解。
