Li L X, Crotty K A, Kril J J, Palmer A A, McCarthy S W
Department of Pathology, University of Sydney, NSW, Australia.
Histochem J. 1999 Apr;31(4):237-40. doi: 10.1023/a:1003528507828.
The effect of melanin bleaching on the immunoreactivity of the MIB1-Ki67 antigen in pigmented melanocytic lesions was investigated. Eight paired non-pigmented and heavily pigmented malignant melanomas (6 primary melanomas and 2 secondary melanomas) were selected. Avidin-biotin immunoperoxidase complex (ABC) and microwave antigen retrieval were used in immunostaining. Sections were incubated with 10% H2O2 for 24h before immunostaining with primary antibody MIB1, or after the completion of immunostaining. Non-bleached controls were obtained by conducting the identical staining but omitting the bleaching procedure. In all heavily pigmented lesions bleached by 10% H2O2 before or after immunostaining, the melanin was bleached effectively and MIB1-positively stained cells were clearly seen. Cell counting in the non-pigmented group found that there were no significant differences in the percentage of MIB1-positive melanoma cells (%MIB1) between non-bleached controls and those sections which had been bleached by 10% H2O2 either before or after the immunostaining. The results suggest that hydrogen peroxide can effectively bleach melanin in pigmented melanocytic lesions without significantly affecting MIB1-Ki67 immunolabelling.
研究了黑色素漂白对色素沉着性黑素细胞病变中MIB1-Ki67抗原免疫反应性的影响。选取了8对非色素沉着性和重度色素沉着性恶性黑色素瘤（6例原发性黑色素瘤和2例继发性黑色素瘤）。免疫染色采用抗生物素蛋白-生物素免疫过氧化物酶复合物（ABC）法及微波抗原修复。切片在与一抗MIB1免疫染色前或免疫染色完成后，用10%过氧化氢孵育24小时。通过进行相同的染色但省略漂白步骤获得未漂白对照。在所有免疫染色前或后用10%过氧化氢漂白的重度色素沉着性病变中，黑色素被有效漂白，且可见MIB1阳性染色细胞。非色素沉着组的细胞计数发现，未漂白对照与免疫染色前或后用10%过氧化氢漂白的切片之间，MIB1阳性黑色素瘤细胞百分比（%MIB1）无显著差异。结果表明，过氧化氢可有效漂白色素沉着性黑素细胞病变中的黑色素，而不显著影响MIB1-Ki67免疫标记。
