Jordan Tierney, Williams Destinee, Criswell Sheila, Wang Yongfu
College of Health Professions, The University of Tennessee Health Science Center, Memphis, TN, USA.
Stowers Institute for Medical Research, Kansas City, MO, USA.
J Histotechnol. 2019 Dec;42(4):177-182. doi: 10.1080/01478885.2019.1649886. Epub 2019 Aug 15.
Melanomas represent the malignant transformation of melanocytes, cells found primarily in the skin to protect epithelium and underlying connective tissues from harmful effects of ultraviolet light. Melanomas vary greatly in morphology and may continue to produce melanin markedly, mildly, or not at all. Performing and evaluating hematoxylin and eosin stains as well as immunohistochemical stains on pigmented melanomas has been a long-standing challenge due to the obscuring pigment. Protocols for removing melanin to reveal cellular morphology have been used successfully for years, but coupling these protocols with stains for immunohistochemistry represents an added challenge. In this study, the investigators evaluated results of various melanin bleaching protocols on tissue morphology, completeness of melanin removal, and immunohistochemistry staining quality. It was found that 1% formamide in 3% HO under bright light without heating outperformed other tested protocols.
黑色素瘤代表黑素细胞的恶性转化，黑素细胞主要存在于皮肤中，以保护上皮组织和下方的结缔组织免受紫外线的有害影响。黑色素瘤的形态差异很大，其产生黑色素的情况可能为显著、轻微或完全不产生。由于色素的遮盖，对色素性黑色素瘤进行苏木精和伊红染色以及免疫组织化学染色并进行评估一直是一项长期挑战。多年来，去除黑色素以显示细胞形态的方案已成功应用，但将这些方案与免疫组织化学染色相结合则是一项额外的挑战。在本研究中，研究人员评估了各种黑色素漂白方案对组织形态、黑色素去除的完整性以及免疫组织化学染色质量的影响。结果发现，在明亮光线下不加热的3%过氧化氢中加入1%甲酰胺的方案优于其他测试方案。
