Qin Xiaoxiao, Lu Yanfen, Peng Zhen, Fan Shuangxi, Yao Yuncong
Technology Industry Group, Beijing Key Laboratory of New Technology in Agricultural Application, National Demonstration Center for Experimental Plant Production Education, Beijing Collaborative Innovation Center for Eco-Environmental Improvement with Forestry Fruit Trees, Plant Science and Technology College, Beijing University of Agriculture, Beijing, China.
Front Chem. 2018 Feb 2;6:9. doi: 10.3389/fchem.2018.00009. eCollection 2018.
The flavonoid system comprises an abundance of compounds with multiple functions; however, their potential synergism in antioxidant function remains unclear. We established an approach using ever-red (RL) and ever-green leaves (GL) of crabapple cultivars during their development to determine interrelationships among flavonoid compounds. RL scored significantly better than GL in terms of the type, composition, and diversity of flavonoids than GL. Principal component analysis predicted flavonoids in RL to have positive interaction effects, and the total antioxidant capacity was significantly higher than the sum of antioxidant capacities of the individual compounds. This synergy was verified by the high antioxidant capacity in rat serum after feeding on red leaves. Our findings suggest that the synergistic effect is a result of the high transcription levels regulated by McMYBs in RL. In summary, individual flavonoids cooperate in a flavonoid system, thus producing a synergistic antioxidant effect, and the approach used herein can provide insights into the roles of flavonoids and other compounds in future studies.
类黄酮系统包含大量具有多种功能的化合物；然而，它们在抗氧化功能方面的潜在协同作用仍不清楚。我们建立了一种方法，利用海棠品种发育过程中的红叶（RL）和绿叶（GL）来确定类黄酮化合物之间的相互关系。在类黄酮的类型、组成和多样性方面，RL的得分显著高于GL。主成分分析预测RL中的类黄酮具有正相互作用效应，且总抗氧化能力显著高于各单一化合物抗氧化能力之和。喂食红叶后大鼠血清中的高抗氧化能力证实了这种协同作用。我们的研究结果表明，这种协同效应是由RL中McMYBs调控的高转录水平所致。总之，单个类黄酮在类黄酮系统中协同作用，从而产生协同抗氧化效应，本文所采用的方法可为未来研究中类黄酮和其他化合物的作用提供见解。
