Ngo Trong Nghia, Vo Cong Thin, Pham Nguyen Kim Tuyen, Phan Nhat Minh, Bui Trong Dat, Ngo Quoc Luan, Dang Van Son, Tran Cong Luan, Mai Dinh Tri, Nguyen Tan Phat
a Graduate University of Science and Technology, Vietnam Academy of Science and Technology , Hanoi , Viet Nam.
b Faculty of Environment and Natural Resources , Kien Giang University , Kien Giang City , Viet Nam.
Nat Prod Res. 2019 Jan;33(2):174-179. doi: 10.1080/14786419.2018.1440227. Epub 2018 Feb 22.
One new cycloartane triterpenoid, named markhacanasin C (1), together with three known triterpenoids, oleanolic acid (2), ursolic acid (3) and 6β,19α-dihydroxyursolic acid (4) were isolated by various chromatographic methods from the most cytotoxic fraction of the ethyl acetate extract of Markhamia stipulata var. canaense V.S. Dang leaves. Among them, 4 was reported for the first time from the genus Markhamia, while 2 and 3 were found for the first time from this species. Their structures were elucidated by IR, UV, HR-ESI-MS and NMR experiments. The cytotoxicity of isolated compounds (3 and 4) against three human cancer cell lines (HeLa, HepG2, and MCF-7) were evaluated. At the concentration of 100 μg/mL, 3 exhibited significant cytotoxic activity (86.36 ± 3.69%).
从云南火焰花（Markhamia stipulata var. canaense V.S. Dang）叶乙酸乙酯提取物的细胞毒性最强部分，通过各种色谱方法分离得到了一种新的环阿尔廷烷三萜类化合物，命名为滇缅火焰花苷C（1），以及三种已知的三萜类化合物，齐墩果酸（2）、熊果酸（3）和6β,19α - 二羟基熊果酸（4）。其中，化合物4是首次从火焰花属中报道，而化合物2和3是首次从该物种中发现。通过红外光谱（IR）、紫外光谱（UV）、高分辨电喷雾电离质谱（HR - ESI - MS）和核磁共振（NMR）实验对它们的结构进行了阐明。评估了分离得到的化合物（3和4）对三种人类癌细胞系（HeLa、HepG2和MCF - 7）的细胞毒性。在浓度为100μg/mL时，化合物3表现出显著的细胞毒性活性（86.36±3.69%）。
