Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi, Vietnam.
Kien Giang University, Kien Giang Province, Vietnam.
Nat Prod Res. 2020 Jul;34(13):1820-1826. doi: 10.1080/14786419.2018.1561686. Epub 2019 Jan 29.
From the leaves of var. V.S. Dang, one new phytosphingolipid, named markhasphingolipid A () together with five known compounds, 4',7--dimethylapigenin (), narigenin (), tectoquinone (), mollic acid (), 1-hexadecanoyl--glycerol were classified by various chromatographic methods. Their structures were designated by IR, UV, HR-ESI-MS, HR-ESI-MS/MS and NMR experiments. All compounds were recognized for the first time from this species. The cytotoxicity of all -hexane fractions and isolated compounds ( & against three human cancer cell lines (HeLa, HepG2, and MCF-7) were evaluated by SRB assay. All -hexane fractions expressed cytotoxic effect on three tested cancer cell lines (at the concentration of 100 μg/mL, percent of cytotoxicity ranged from 55.81% to 95.83%) as well as compound (IC ranged from 48.51 to 63.30 μM) whereas fraction H.I and compound did not show activity.[Formula: see text].
从 var. V.S. Dang 的叶子中，分离得到了一个新的植物神经酰胺，命名为 markhasphingolipid A ()，并与五个已知化合物一起分离得到，分别为 4',7--二甲氧基芹菜素 ()、柚皮素 ()、 tectoquinone ()、莫利酸 () 和 1-十六酰基--甘油 。采用各种色谱方法对它们的结构进行了鉴定。通过 IR、UV、HR-ESI-MS、HR-ESI-MS/MS 和 NMR 实验确定了它们的结构。所有化合物均首次从该种植物中分离得到。采用 SRB 法评价了所有正己烷馏分和分离得到的化合物（&）对三种人癌细胞系（HeLa、HepG2 和 MCF-7）的细胞毒性。所有正己烷馏分在三种测试的癌细胞系中均表现出细胞毒性作用（在 100μg/mL 的浓度下，细胞毒性百分比范围为 55.81%至 95.83%），以及化合物 （IC 范围为 48.51 至 63.30μM），而馏分 H.I 和化合物 则没有活性。
