Biochemistry Department, Faculty of Science, Al Faisalia, King Abdulaziz University, Jeddah, Saudi Arabia.
Medical Research Division, Narcotics, Ergogenic Aids and Poisons Department, National Research Centre, El-Bohouth Street, Dokki, Giza, 12622, Egypt.
Inflammopharmacology. 2018 Aug;26(4):963-972. doi: 10.1007/s10787-018-0456-2. Epub 2018 Feb 22.
Temporal lobe epilepsy (TLE) is present in 30% of epileptic patients and does not respond to conventional treatments. Bone marrow derived mesenchymal stem cells (BMSCs) induce endogenous neural stem cells, inhibit neurodegeneration, and promote brain self-repair mechanisms. The present study addresses the feasibility of BMSCs transplantation against pilocarpine-induced TLE experimentally. BMSCs were injected either intravenously (IV) or in hippocampus bilaterally (IC). Increased cell count of BMSCs was achieved via IC route. BMSCs treatment ameliorated the pilocarpine-induced neurochemical and histological changes, retained amino acid neurotransmitters to the normal level, downregulated the immunoreactivity to insulin growth factor-1 receptor, synaptophysin, and caspase-3 and reduced oxidative insult and inflammatory markers detected in epileptic model. It is worth noting that BMSCs IC-administered showed more pronounced effects than those administered via IV route. BMSCs transplantation presents a promise for TLE treatment that has to be elucidated clinically.
颞叶癫痫（TLE）存在于 30%的癫痫患者中，对常规治疗无反应。骨髓间充质干细胞（BMSCs）可诱导内源性神经干细胞，抑制神经退行性变，促进大脑自我修复机制。本研究旨在探讨 BMSCs 移植治疗匹鲁卡品诱导的 TLE 的可行性。BMSCs 通过静脉内（IV）或双侧海马内（IC）注射。通过 IC 途径可增加 BMSCs 的细胞计数。BMSCs 治疗改善了匹鲁卡品诱导的神经化学和组织学变化，将氨基酸神经递质维持在正常水平，下调胰岛素样生长因子-1 受体、突触素和半胱天冬酶-3 的免疫反应性，并降低癫痫模型中检测到的氧化损伤和炎症标志物。值得注意的是，IC 给予 BMSCs 比 IV 途径给予 BMSCs 更能显著发挥作用。BMSCs 移植为 TLE 治疗提供了一种有前途的方法，有待临床阐明。
