Doly M, Droy-Lefaix M T, Bonhomme B, Braquet P
Presse Med. 1986 Sep 25;15(31):1480-3.
The purposes of the experimental work presented here were to ascertain that diabetes causes impairment of the visual function and to test the protective properties of Ginkgo biloba extract against this impairment. The experimental method selected was the isolated albino rat retina maintained in survival by perfusion. Retinal function was evaluated by electroretinographic recording in response to a light stimulus. The rats were made diabetics by injection of alloxan. After one month of diabetes the electroretinogram amplitude was significantly decreased in diabetic rats as compared with control animals. This decrease in amplitude was more pronounced after 2 months of diabetes, which confirmed that retinal function was worse. In rats treated with Ginkgo biloba extract, after 2 months of diabetes the electroretinograms had a significantly greater amplitude that that observed in untreated rats. These results are attributed to the specific role played by free oxygenated radicals on the retina of diabetic rats and to the free radical scavenger property of Ginkgo biloba extract.
本文所展示的实验工作目的是确定糖尿病会导致视觉功能受损，并测试银杏叶提取物对这种损伤的保护作用。所选的实验方法是通过灌注维持存活的离体白化大鼠视网膜。通过对光刺激的视网膜电图记录来评估视网膜功能。通过注射四氧嘧啶使大鼠患糖尿病。糖尿病一个月后，与对照动物相比，糖尿病大鼠的视网膜电图振幅显著降低。糖尿病两个月后，这种振幅降低更为明显，这证实了视网膜功能更差。在用银杏叶提取物治疗的大鼠中，糖尿病两个月后，视网膜电图的振幅明显大于未治疗大鼠。这些结果归因于游离氧自由基在糖尿病大鼠视网膜上所起的特定作用以及银杏叶提取物的自由基清除特性。
