Livestock Center Oberschleissheim, Veterinary Faculty of the Ludwig-Maximilians-University Munich, Germany.
Boehringer Ingelheim Animal Health GmbH, Germany.
Vaccine. 2018 Mar 20;36(13):1789-1795. doi: 10.1016/j.vaccine.2018.02.025. Epub 2018 Feb 19.
Safety testing is one major part of the licensing procedure for veterinary vaccines and demands a large number of animals. Magnetic resonance imaging (MRI) was tested as an alternative, which may lead to a reduction in numbers of animals required for safety testing, and, correspondingly to a detailed description of the three-dimensional extent of the local tissue reaction repetitively in live pigs. In previous pig studies the following questions arose:To answer these questions the following study was performed by comparing two vaccine groups of suckling piglets (8 animals per group; A and B) with two control groups (4 animals per group; C and D). One control group was injected with a saline solution (C) and the other was only tattoo marked (D). The animals were examined using MRI at days 1, 8, 15, 22, 29, 36, and 43 post vaccination, ending with a final pathomorphologic examination. Pathomorphologic examination confirmed MRI findings. Saline solution does not result in a local tissue reaction as detected after injecting vaccines. Tattoo marking causes no local tissue reaction, neither in MRI nor in pathomorphologic examination. Therefore, MRI can be used as an alternative method for safety testing of vaccines in pigs of different age categories offering repetitive measurements of local tissue reactions. Involved cells might be examined only in a final pathomorphologic examination at the end of the trial on a reduced number of animals.
安全性测试是兽医疫苗许可程序的主要部分之一，需要大量动物。磁共振成像（MRI）已被测试为替代方法，这可能会减少安全性测试所需的动物数量，并相应地更详细地描述活体猪中局部组织反应的三维范围。在以前的猪研究中出现了以下问题：
为了回答这些问题，我们进行了一项研究，比较了两组哺乳期仔猪（每组 8 只；A 和 B）和两组对照组（每组 4 只；C 和 D）。一组对照猪只注射生理盐水（C），另一组仅纹身标记（D）。动物在接种疫苗后第 1、8、15、22、29、36 和 43 天接受 MRI 检查，最后进行最终的病理形态学检查。病理形态学检查证实了 MRI 的发现。与注射疫苗后检测到的局部组织反应不同，生理盐水不会导致局部组织反应。纹身标记既不会在 MRI 检查中，也不会在病理形态学检查中引起局部组织反应。因此，MRI 可作为不同年龄段猪疫苗安全性测试的替代方法，可重复测量局部组织反应。在试验结束时，在减少的动物数量上，仅在最终的病理形态学检查中检查参与细胞。
