Fu Siying, Duan Yujie, Wang Siqi, Ren Yipeng, Bu Wenjun
Institute of Entomology, College of Life Sciences, Nankai University, Tianjin 300071, China.
Insects. 2021 Mar 5;12(3):226. doi: 10.3390/insects12030226.
(Hemiptera: Alydidae) is a major agricultural pest in East Asia that causes considerable economic losses to the soybean crop each year. However, the molecular mechanisms governing the growth and development of have not been fully elucidated. In this study, the Illumina HiSeq6000 platform was employed to perform de novo transcriptome assembly and determine the gene expression profiles of this species across all developmental stages, including eggs, first-, second-, third-, fourth-, and fifth-instar nymphs, and adults. In this study, a total of 60,058 unigenes were assembled from numerous raw reads, exhibiting an N50 length of 2126 bp and an average length of 1199 bp, and the unigenes were annotated and classified with various databases, such as the Kyoto Encyclopedia of Genes and Genomes (KEGG), Clusters of Orthologous Groups (COG), and Gene Ontology (GO). Furthermore, various numbers of differentially expressed genes (DEGs) were calculated through pairwise comparisons of all life stages, and some of these DEGs were associated with immunity, metabolism, and development by GO and KEGG enrichment. In addition, 35,158 simple sequence repeats (SSRs) and 715,604 potential single nucleotide polymorphisms (SNPs) were identified from the seven transcriptome libraries of . Finally, we identified and summarized ten wing formation-related signaling pathways, and the molecular properties and expression levels of five wing development-related genes were analyzed using quantitative real-time PCR for all developmental stages of . Taken together, the results of this study may establish a foundation for future research investigating developmental processes and wing formation in hemimetabolous insects and may provide valuable data for pest control efforts attempting to reduce the economic damage caused by this pest.
（半翅目：缘蝽科）是东亚地区一种主要的农业害虫，每年给大豆作物造成相当大的经济损失。然而，其生长发育的分子机制尚未完全阐明。在本研究中，利用Illumina HiSeq6000平台进行从头转录组组装，并确定该物种在所有发育阶段（包括卵、一龄、二龄、三龄、四龄和五龄若虫以及成虫）的基因表达谱。本研究从大量原始 reads 中总共组装出60,058个单基因，N50长度为2126 bp，平均长度为1199 bp，这些单基因通过各种数据库（如京都基因与基因组百科全书（KEGG）、直系同源簇（COG）和基因本体论（GO））进行注释和分类。此外，通过对所有生命阶段进行成对比较计算出不同数量的差异表达基因（DEG），其中一些DEG通过GO和KEGG富集与免疫、代谢和发育相关。此外，从该物种的七个转录组文库中鉴定出35,158个简单序列重复（SSR）和715,604个潜在的单核苷酸多态性（SNP）。最后，我们鉴定并总结了十条与翅形成相关的信号通路，并使用定量实时PCR分析了该物种所有发育阶段五个与翅发育相关基因的分子特性和表达水平。综上所述，本研究结果可为今后研究半变态昆虫的发育过程和翅形成奠定基础，并可为试图减少该害虫造成的经济损失的害虫防治工作提供有价值的数据。
