利用转录组分析鉴定和描述美洲芫菁（鞘翅目：芫菁科）中的气味结合蛋白。
Transcriptome analysis used to identify and characterize odorant binding proteins in Agasicles hygrophila (Coleoptera: Chryspmelidae).
机构信息
Laboratory of Insect Behavior and Evolutionary Ecology, College of Life Science and Technology, Central South University of Forestry and Technology (CSUFT), Changsha, China.
Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China.
出版信息
J Insect Sci. 2023 Sep 1;23(5). doi: 10.1093/jisesa/iead081.
The transcriptomes of Agasicles hygrophila eggs and first instar larvae were analyzed to explore the olfactory mechanism of larval behavior. The analysis resulted in 135,359 unigenes and the identification of 38 odorant-binding proteins (OBPs), including 23 Minus-C OBPs, 8 Plus-C OBPs, and 7 Classic OBPs. Further analysis of differentially expressed genes (DEGs) revealed 10 DEG OBPs, with 5 (AhygOBP5, AhygOBP9, AhygOBP12, AhygOBP15 and AhygOBP36) up-regulated in first instar larvae. Verification of expression patterns of these 5 AhygOBPs using qPCR showed that AhygOBP9 and AhygOBP36 were mainly expressed in the adult stage with gradually increasing expression in the larval stage. AhygOBP5, AhygOBP12, and AhygOBP15 were not expressed in eggs and pupae, and their expression in larvae and adults showed no clear pattern. These 5 AhygOBPs may play an olfactory role in larval behavior, providing a basis for further investigation of their specific functions and clarifying the olfactory mechanism of A. hygrophila.
对美洲大蠊卵和一龄幼虫的转录组进行了分析，以探讨幼虫行为的嗅觉机制。分析得到了 135359 条非编码 RNA 序列，并鉴定了 38 种气味结合蛋白（OBP），包括 23 种 Minus-C OBPs、8 种 Plus-C OBPs 和 7 种 Classic OBPs。对差异表达基因（DEGs）的进一步分析显示，有 10 个 DEG OBPs，其中 5 个（AhygOBP5、AhygOBP9、AhygOBP12、AhygOBP15 和 AhygOBP36）在一龄幼虫中上调表达。使用 qPCR 对这 5 个 AhygOBPs 的表达模式进行验证，结果表明 AhygOBP9 和 AhygOBP36 主要在成虫期表达，在幼虫期表达逐渐增加。AhygOBP5、AhygOBP12 和 AhygOBP15 在卵和蛹期不表达，其在幼虫和成虫期的表达没有明显的模式。这 5 个 AhygOBPs 可能在幼虫行为的嗅觉中发挥作用，为进一步研究它们的特定功能和阐明美洲大蠊的嗅觉机制提供了依据。
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