Hermiston Agricultural Research and Extension Center, Oregon State University, Hermiston, OR, United States of America.
Department of Crop and Soil Science, Oregon State University, Corvallis, OR, United States of America.
PLoS One. 2018 Feb 23;13(2):e0193415. doi: 10.1371/journal.pone.0193415. eCollection 2018.
Micronutrient deficiency, also known as the hidden hunger, affects over two billion people worldwide. Potato is the third most consumed food crops in the world, and is therefore a fundamental element of food security for millions of people. Increasing the amount of micronutrients in food crop could help alleviate worldwide micronutrient malnutrition. In the present study, we report on the identification of single nucleotide polymorphism (SNP) markers associated with folate, an essential micronutrient in the human diet. A high folate diploid clone Fol 1.6 from the wild potato relative Solanum boliviense (PI 597736) was crossed with a low/medium folate diploid S. tuberosum clone USW4self#3. The resulting F1 progeny was intermated to generate an F2 population, and tubers from 94 F2 individuals were harvested for folate analysis and SNP genotyping using a SolCap 12K Potato SNP array. Folate content in the progeny ranged from 304 to 2,952 ng g-1 dry weight. 6,759 high quality SNPs containing 4,174 (62%) polymorphic and 2,585 (38%) monomorphic SNPs were used to investigate marker-trait association. Association analysis was performed using two different approaches: survey SNP-trait association (SSTA) and SNP-trait association (STA). A total of 497 significant SNPs were identified, 489 by SSTA analysis and 43 by STA analysis. Markers identified by SSTA were located on all twelve chromosomes while those identified by STA were confined to chromosomes 2, 4, and 6. Eighteen of the significant SNPs were located within or in close proximity to folate metabolism-related genes. Forty two SNPs were identical between SSTA and STA analyses. These SNPs have potential to be used in marker-assisted selection for breeding high folate potato varieties.
微量营养素缺乏，也被称为隐性饥饿，影响着全球超过 20 亿人。土豆是世界上第三大消费的粮食作物，因此是数百万人粮食安全的基本要素。增加粮食作物中的微量营养素含量有助于缓解全球微量营养素营养不良问题。在本研究中，我们报告了与叶酸相关的单核苷酸多态性 (SNP) 标记的鉴定，叶酸是人类饮食中必不可少的微量营养素。从野生马铃薯近缘种 Solanum boliviense（PI 597736）中分离出高叶酸二倍体克隆 Fol 1.6，与低/中叶酸二倍体 S. tuberosum 克隆 USW4self#3 杂交。将产生的 F1 后代进行互交，产生 F2 群体，并对来自 94 个 F2 个体的块茎进行叶酸分析和 SNP 基因分型，使用 SolCap 12K 马铃薯 SNP 阵列。后代的叶酸含量范围为 304 至 2952ng g-1 干重。使用 6759 个高质量 SNP 进行标记-性状关联分析，其中包含 4174 个（62%）多态性和 2585 个（38%）单态性 SNP。使用两种不同的方法进行关联分析：调查 SNP-性状关联 (SSTA) 和 SNP-性状关联 (STA)。共鉴定出 497 个显著 SNP，其中 489 个通过 SSTA 分析鉴定，43 个通过 STA 分析鉴定。通过 SSTA 鉴定的标记位于 12 条染色体上，而通过 STA 鉴定的标记仅限于染色体 2、4 和 6。18 个显著 SNP 位于叶酸代谢相关基因内或附近。42 个 SNP 在 SSTA 和 STA 分析中是相同的。这些 SNP 有可能用于辅助选择高叶酸马铃薯品种的选育。
